摘要
目的观察肺动脉内皮细胞血小板源性生长因子（ＰＤＧＦ）旁分泌及其调节在缺氧性肺动脉高压中所起的作用。方法采用核酸分子杂交技术，分析缺氧时小牛肺动脉内皮细胞（ＰＡＥＣ）表达ＰＤＧＦ－Ａ链、－Ｂ链ｍＲＮＡ的变化及外源一氧化氮（ＮＯ）对其表达的调节作用。结果缺氧（２．５％Ｏ２和０％Ｏ２）早期（１．５，３ｈ）能非常显著地增加肺动脉内皮细胞ＰＤＧＦ－Ａ，ＰＤＧＦ－Ｂ链ｍＲＮＡ的表达（Ｐ＜０．００１，ｖｓ常氧组）；缺氧６～４８ｈ与常氧组相比ＰＤＧＦ－Ａ链无明显差别，ＰＤＧＦ－Ｂ链ｍＲＮＡ表达增加（Ｐ＜０．０５）。外源ＮＯ能非常显著地降低常氧肺动脉内皮细胞ＰＤＧＦ－Ａ链（Ｐ＜０．０１）和常氧ＰＤＧＦ－Ｂ链（Ｐ＜０．００１）ｍＲＮＡ的表达水平，而不能明显地抑制缺氧ＰＤＧＦ－Ａ，－Ｂ链的表达。结论缺氧可能一方面通过刺激肺动脉内皮细胞ＰＤＧＦ基因表达加强，一方面减弱ＮＯ对ＰＤＧＦ基因表达的抑制作用，从而参与缺氧性肺动脉高压的肺血管收缩反应加强和肺血管结构改建的发生过程。
Aim\ To investigate the role of platelet derived growth factor(PDGF) of pulmonary arterial endothelial cells(PAEC) under hypoxic condition may play important roles in the development of hypoxic pulmonary artery hypertension(HPAH).\ Methods\ The effects of hypoxia on PDGF gene expression in new bovine PAEC and the modulation by nitric oxide(NO) were measured by dot blot hybridization.\ Result\ PDGF A, B chain mRNA expression increased significantly when PAECs were incubated under 0% and 2 5% O 2 hypoxic condition for 1 5￣3 h( P<0 01, vs 21% O 2 control).\ PDGF B chain mRNA kept on high level till 48 h( P < 0 05), whereas, PDGF A chain mRNA did not increase significantly during 6￣48 h.\ Exogeneous NO donor(SIN 1) significantly inhibited PDGF A chain( P <0 01) and PDGF B chain( P <0 001) mRNA expression under normoxic condition(21%O 2), but not inhibit under hypoxic condition(2 5% O 2 and 0% O 2 ).\ Conclusion\ These results show that hypoxia may stimulate the expression of PDGF in PAEC in vitro and the inhibition of PDGF mRNA expression by NO under 21%O 2 condition is eliminated by hypoxia, which may lead to the development of HPAH.
出处
《高血压杂志》
CSCD
1997年第2期100-103,共4页
Chinese Journal of Hypertension
基金
国家自然科学基金
关键词
缺氧
肺动脉内皮细胞
一氧化氮
PDGF
hypoxia
pulmonary artery endothelial cell
platelet derived growth factor
nitric oxide
