摘要
用单一饱和剂量分析法(下称单点法)检测了42例人乳腺癌组织雌二醇受体(ER)的容量,每例肿瘤组织同时用葡聚糖包裹炭末法(下称多点法)作对照检查。单点法阳性检出率为59.5％,多点法45.24％,两者无显著差异(P>0。1)。在单点法阳性的25例中有16例、阴性的17例中有14例与多点法检出结果一致,总符合率为71·4％。受体平均结合容量分别为38.22±18和27.91±28fmol/mg蛋白,两者无显著差异(P>0·3),两种分析法的定量有非常显著相关(r=0.6981,P<O.001),认为单点法经实践取得经验后可用于临床检查。
The estradiol receptor (ER) level of 42 biopsies in human breast cancer tissue was estimated by the DCC method. Each of the tumor biopsies in 42 cases was simultaneously assayed by using both a single point and a multiple point assay of DCC method. The results showed that a detectable rate of ER positive is 59.52% by the single point assay and is 45.24% by a multiple point assay. No significant difference between these results was found by x2 test (P>0.1). For the single point assay, 16 ER positive and 14 ER negative accorded with the results by the multiple point assay. The total percentage in coincidence with ER positive and ER negative of the multiple point assay is 71.4%. An average binding capacity of ER is 38.22±6.18 fmol/mg cytosol protein using the single point method, and is 27.91 ± 5.28 fmol/mg protein using by the multiple point method. Both of them have no significant difference (P>0.3). A quantitative correlation of receptor level between the 2 assays is very remarkable (r=0.6981 P<0.001). We consider that the single point assay might be used clinically after obtaining experience which was assayed by the single point assay as compared to the multiple point assay in given numbers of human breast cancer tissue.
出处
《中山医科大学学报》
CSCD
1991年第2期120-122,共3页
Academic Journal of Sun Yat-sen University of Medical Sciences
关键词
乳腺癌
雌二醇受体
单点法
Estradiol receptor Single saturation dose method Dextran-coated charcoal method
