摘要
目的 :观察丹参对新生期大鼠缺氧缺血性脑损伤 (HIBI)后不同时间血浆、桥延脑神经肽Y1 36 和降钙素基因相关肽免疫活性物质 (ir NPY、ir CGRP)含量的影响。方法 :7日龄大鼠随机分为HIBI组(A组 )、HIBI加丹参治疗组 (B组 )和假手术组 (C组 ) ,每组按不同时间各分成 4个小组。在新生期大鼠HI BI后即刻及其后每 12h向腹腔注射丹参注射液 ,每次 0 5ml。采用放射免疫分析法观察HIBI后即刻、12、2 4、4 8h血浆和桥延脑ir NPY、ir CGRP含量的变化。结果 :HIBI后不同时间血浆ir NPY、ir CGRP含量均显著升高 ;桥延脑ir CGRP、ir NPY含量不同程度升高或降低。应用丹参注射液后血浆、桥延脑ir NPY水平的上升被部分逆转。结论 :中枢及外周神经肽Y1 36 和降钙素基因相关肽参与HIBI的病理生理过程 ;丹参部分逆转新生大鼠HIBI后ir NPY水平的异常升高 ,可能是丹参促进损伤后脑功能恢复的作用途径之一。
Objective: To observe the effects of Salvia miltiorrhiza (SM) on levels of neuropeptide Y 1 36 and calcitonin gene related peptide immune reactive substances (ir NPY?ir CGRP) in blood plasma and pons oblongata after hypoxia ischemic brain injury (HIBI) in neonatal rats. Methods: Seven day old rats were randomized into HIBI group (A), HIBI+SM group (B) and sham operation group(C). And each group was subdivided into 4 subgroups according to the different time after operation. 0.5 ml SM was injected intraperitoneally immediately and every 12 hrs afterwards. Changes of ir NPY and ir CGRP levels in plasma and pons oblongata were observed immediately and 12, 24 and 48 hrs after HIBI by radioimmunoassay. Results: Plasma levels of ir NPY and ir CGRP in different times after HIBI were all significantly raised but those in pons oblongata were either raised or lowered to a certain degree. Part of the elevated ir NPY could be reversed by SM injection. Conclusion: Central and peripheral neuropeptide Y 1 36 and calcitonin gene related peptide take part in the pathophysiological process of HIBI, SM could partially reverse the abnormal post HIBI elevation of ir NPY, which may be one of the pathways of SM in promoting recovery of damaged brain function.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2002年第8期607-609,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
全军"九.五"医学科研规划课题 (No.98D0 2 2 )
