摘要
目的 对胃粘膜相关淋巴组织( MALT) 淋巴瘤浸润子宫平滑肌瘤病理形态进行定量测定研究。方法 对瘤组织采用常规石蜡切片、HE 染色及ABC 法免疫组化染色, 并运用HPIAS- 1000 彩色病理图文分析系统对肿瘤细胞核的形态进行定量测定。结果 胃粘膜、粘膜下层、肌层及平滑肌瘤内均可见弥漫多量密集成片的中心细胞样瘤细胞浸润, 在胃粘膜内并可见淋巴上皮病变。定量测定的结果表明胃、淋巴结及子宫肌瘤内淋巴瘤细胞细胞核15 项形态参数与对照组间除一项参数外( 胃淋巴瘤细胞与对照组间细胞核的椭圆度) , 所有的指标均有高度显著性差异( P< 0-001) 。结论 MALT 淋巴瘤细胞与正常淋巴细胞形态学参数之间确实具有差异, 定量测定的方法要比用肉眼在光镜下直接观察更为敏感, 其对于MALT 淋巴瘤疑难病例的诊断提供了新的诊断方法,
Objective To investigate the quantitative morphometric significance of the mucosa associated lymphoid tissue (MALT) lymphoma in stomach and leiomyoma of uterus. Methods The routine paraffin slides were made, stained with HE and immunochemically by ABC method. Then, the morphologic appearance of the nuclei of lymphoma cells were measured by using the HPIAS-1000 color pathology words and picture analysis system. Results In gastric mucosa, submucosa and the muscle layer of smooth and the uterine leiomyoma, there were diffusive and dense infiltrations of centrocyte like cells, and the lymphoepithelial lesions could be seen in the mucosa of stomach. The morphologic measurement showed great significant differences (P<0.0001) for the 15 items of morphology parameters (except one, the ellips degree between the MALTlymphoma lymphocytes in stomach and normal control lymphocytes, between the MALT nuclei of lymphoma lymphocytes in stomach, lymph node and leiomyoma and those of normal control lymphocytes. Conclusions There may be indeed some differences of morphology parameters of MALT lymphoma and normal control lymphocytes. The quantitative morphometry was more sensitive than the direct observation under microscope by naked eyes. It offers us a new diagnostic method for the difficult cases of MALT lymphoma and, therefore, it may be capable of important clinical significance.
出处
《中国体视学与图像分析》
1999年第4期198-201,I001,共5页
Chinese Journal of Stereology and Image Analysis
基金
国家教委留学回国人员科研启动基金!( 教外司留[1997]436 号)
南京市留学回国人员科研基金
