摘要
从青贮玉米(Zea mays)以及新鲜泡菜中分离乳酸菌,通过初筛和复筛获得1株产酸能力较强的耐低温乳酸菌L2;对其进行了形态学描述和生理生化鉴定,对照伯杰式手册确定其种属;采用Box-Behnken实验设计进行培养条件优化实验,确定了该菌的最佳发酵条件。结果表明,该菌的最佳发酵条件为接种量3.1%、初始pH 6.4、培养温度18.4℃。
One lactic acid bacteria strain L2with low temperature resistant and more lactic acid production was isolated and selected from corn silage and fresh kimchi.The strain was identified with morphological,physiological and biochemical characterization according to the Bergey′s manual of systematic bacteriology.The culture conditions of this strain were optimized as 3.1%(v/v)inoculation amount,initial pH 6.4and temperature of 18.4℃ with Box-Behnken Design.
出处
《草业科学》
CAS
CSCD
北大核心
2014年第7期1380-1387,共8页
Pratacultural Science
基金
青海高原放牧家畜营养与生态国家实验室培育基地项目基金
关键词
耐低温乳酸菌
分离
优化培养
low temperature lactic acid bacteria
isolation
optimization of culture
