摘要
目的研究miR-10b在肝细胞癌中的表达及对肝癌细胞增殖,侵袭和转移的影响。方法荧光实时定量PCR检测肝癌组织10例,癌旁组织10例及正常肝组织5例miR-10b的表达,分析miR-10b与肝癌临床病理特征的关系;观察人肝癌细胞株Hep G2下调miR-10b表达后对细胞增殖,侵袭和转移的影响。结果 MiR-10b在癌组织组与癌旁组织组,癌组织组与正常组织组表达差异有统计学意义(P <0. 05或<0. 01),而癌旁组织组与正常组织组的表达差异无统计学意义(P> 0. 05)。抑制miR-10b可以明显抑制人肝癌细胞株的增殖和克隆能力(P <0. 01),并可以减弱其抵抗凋亡的能力(P <0. 01)。抑制miR-10b的表达可以明显减弱肝细胞癌株的侵袭和转移能力(P <0. 01)。结论MiR-10b在临床上可以作为一种新的恶性肿瘤的生物检测标识,具有很大的临床应用价值。
Objective To investigate the expression and clinical significance of nfiR-10b in primary hepatocellular carcinoma （HCC） , and to explore the effects of nfiR-10b on cell proliferation,invasion and metastasis in HCC. Methods The expression levels of nfiR-10b were detected by real-fime reverse transcription polymerase chain reaction （Real-time PCR） in 10 cases of primary hepatocellular carcinoma tissues,20 cases of adjacent tissues and 10 cases of normal liver tissues,and the correlation between the expression levels of nfiR-10b and clinicopathological features of HCC was analyzed. Moreover the effects of miR-10b on cell proliferation,invasion and metastasis in HCC after the transfect synthetic miR-10b inhibitor （anti- nfiR-10b） was transfected into HepG2 cells to inhabit the expression of nfiR-10b in cells were observed and analyzed. Results There were significant differences in the expression levels of nfiR-10b among primary hepatocellular carcinoma tissues and cancer adjacent tissues and between primary hepatocellular carcinoma tissues and normal liver tissues （P 〈 0.05, or P 〈 0.01 ）. However there were no significant differences in the expression levels of miR-10b between cancer adjacent tissues and normal liver tissues（ P 〉 0.05 ）. The inhibition of miR-10b expression could repress the abilities of proliferation and cloning formation of human hepatoma carcinoma cell strain （P 〈 0.01 ） , moreover, which could weaken its ability- to resist apoptosis of human hepatoma cell lines-HepG2（P 〈 0.01 ）. In addition the inhibition of the expression of miR-10b could also decrease significantly the invasion and metastasis ability of human hepatoma cell lines-HepG2 （ P 〈 0.01 ）. Conclusion MiR-10b can be regarded as an new biological detection marker in clinic, it is of great value in clinical practice.
作者
王子承
田秉璋
毛先海
段小辉
杨建辉
周力学
WANG Zicheng;TIAN Bingzhang;MAO Xianhai(Department of Hepatobiliary Pancreatic Surgery,Hunan Provicial People＇s Hospital,Hunan,Changsha 410005,China)
出处
《河北医药》
CAS
2018年第24期3685-3690,3695,共7页
Hebei Medical Journal
关键词
MIR-10B
原发性肝细胞癌
临床意义
增殖
转移
miR-10b
primary hepatocellular carcinoma
clinical significance
proliferation
metastasis
