摘要
目的:探讨喉鳞状细胞癌组织miRNA表达。方法:对我院6例手术并经病理确诊的喉鳞状细胞癌患者,及6例良性病变患者,提取喉部组织,应用miRNA基因芯片检测两组之间不同miRNA水平的表达情况。结果:与对照组相比,喉鳞状细胞癌组织中表达升高的有,miR-200b、miR-21、let-7i、let-7c、miR-93、miR19a、miR-106b和miR19b-2;下调的有,miR-543、miR-520h、miR-582-3p、miR-1305和miR-203。未见或鲜见报道的有miR-1305、miR-203、miR19a和miR19b-2。结论:喉鳞状细胞癌中存在miRNA的表达失调,为进一步的功能学研究和扩大实验提供了线索。
OBJECTIVE：To investigate the significance of microRNAs （miRNA） in patients with laryngeal squamous cell carcinoma （LSCC）.METHODS：Six patients who suffered laryngeal squamous cell carcinoma and 6 patients who had benign lesions were selected and went through operation.Laryngeal tissues were sampled and Global miRNA profilings were conducted on miRNA chips.RESULTS：Several miRNAs were significantly differentially expressed between malignant versus benign tissues.Overexpression of miR-200b,miR-21,let-7i,let-7c,miR-93,miRNA19a,miR-106b,miR19b-2 and underexpression of miR-543,miR-520h,miR-582-3p,miR-1305 and miR-203 were detected.Among them,miR19a,miR19b-2 and miR-203 were rarely reported and miR-1305 was never reported.CONCLUSION：A group of aberrantly expressed miRNAs in LSCC which provided insights for future investigations were identified.
出处
《中华肿瘤防治杂志》
CAS
2010年第14期1073-1076,共4页
Chinese Journal of Cancer Prevention and Treatment
关键词
喉肿瘤
微RNA
基因表达调控
laryngeal neoplasms
microRNA
gene expression regulation
