摘要
目的探讨熊猴类芽孢杆菌侵入人体能能否致病及其他。方法对病例所在地现场开展流行病学调查;组织临床医师进行座谈,收集病例临床资料及相关实验室材料。结果将病例关节液初始培养出的细菌进行选择性培养,从已知文献中未查找到PyrA阳性与土拉弗朗西斯菌的相关性,对分离出的细菌进行16SrRNA扩增、测序对比等检测,在NCBI网站用Blast进行对比,获得同源性最高的微生物是熊猴类芽孢杆菌,最后判定为1例熊猴类芽孢杆菌感染者。讨论当地熊猴类芽孢杆菌的来源,病例关节肿胀久治不愈是否是该菌所致,此菌在当地首次从人体组织液中培养出来,对当地政府提出了新的机遇和挑战。
Objectives To investigate whether Paenibacillus assamensis could invade human body and would be pathogenic. Methods An epidemiological investigation was carried out on the location of the case by an informal discussion with local clinicians and collecting clinical and laboratory data. The initial cultured bacteria from the synovia of joints were selectively cultured. Results The correlation between PyrA positive and Francisellatularensis was not found from known literatures; the isolated bacteria were then amplified by 16 SrRNA, sequenced and compared. Compared with the Blast in NBCI Web site, the microorganism isolated had a high homology with Paenibacillus assamensis, and finally an infection of Paenibacillus assamensis in this case was diagnosed. Conclusions What was the source of local Paenibacillus assamensis and whether the joint inflammation of the case was caused by the bacteria. Paenibacillus assamensis was isolated for the first time from the tissue fluid of local people. It would be a new opportunity and challenge for local government.
作者
汤志刚
陆洪潮
袁华芳
TANG Zhigang;LU Hongchao;YUAN Huafang
出处
《环境卫生学杂志》
2018年第5期437-440,共4页
JOURNAL OF ENVIRONMENTAL HYGIENE
关键词
土拉弗朗西斯菌熊猴类芽孢杆菌
机遇
挑战
francisella tularensis paenibacillus assamensis
opportunity
challenge
