摘要
目的探讨当归多糖(ASP)对D-半乳糖(D-gal)致小鼠胰腺损伤的保护作用。方法雄性C57BL/6 J小鼠随机分为3组,每组10只。D-gal组:小鼠皮下注射D-gal (120 mg/kg, qd×42 d); ASP+D-gal组:D-gal注射时间与剂量同D-gal模型组,从模型复制第16天起,腹腔注射ASP(40 mg/kg,qd×27 d);正常对照组:小鼠皮下注射等量与等时的生理盐水。各组给药完成后第2天检测相关指标,取外周血检测空腹血糖含量并行口服葡萄糖耐量测定(OGTT);取胰腺称湿重计算脏器指数;制备石蜡切片,HE染色观察胰腺组织病理结构,图像分析法计数胰岛内有核细胞数和相关面积;制备胰腺组织匀浆,检测丙二醛(MDA)含量和总抗氧化能力(T-AOC)。结果从注射D-gal第16天开始,腹腔注射ASP共27 d(ASP+D-gal组),小鼠胰腺湿重与脏器指数明显降低,组织病理损伤减轻;空腹血糖显著下降;在30 min和120 min的OGTT水平差别不明显;OGTT曲线下面积(AUC)降低;胰岛内有核细胞数减少,单个细胞面积减小;T-AOC活性上升,MDA含量下降。结论 D-gal可致胰腺结构损伤和功能衰退,ASP能拮抗D-gal从而减轻对胰腺的损伤,其机制可能与减轻氧化损伤有关。
Objective To explore the protective effect of Angelica sinensis polysaccharide （ASP）on murine pancreas induced by the D-galactose （D-gal）. Methods The male C57BL/6J mice were randomly divided into 3 groups （ 10 mice in each group） ： D-gal group in which mice were received hypodermic injection of D-gal （ 120 mg/kg, qd x 42 days） , ASP ＋ D-gal group in which the dose and time of the injection of D-gal were the same as that in the D-gal group, from the 16th days of the injection of D-gal, mice intraperitoneally injected ASP（40 mg/kg, qd x 27 days）, and normal control group in which mice received the same dose and time of saline. The related indicators were detected on the second day after modeling and administration. Fasting blood glucose （FBG） , oral glucose tolerance test （OGTT） and fasting insulin level were measured by collecting peripheral blood. Pancreas weight and visceral index were calculated. Paraffin sections were prepared. After HE staining, the micro-structures of the pancreas of each group were observed. The mononuclear cells and the related area in islets were detected by using image analysis method. Malonaldehyde （MDA） and total antioxidant capacity （T-AOC） were analyzed by preparing pancreas tissue homogenate. Results At the beginning of the 16th day of the injection of D-gal, mice were intraperitoneally injected ASP in 27 days. Compared with the control group, pancreatic wet weight and visceral index of ASP ＋ D-gal mice decreased significantly. The FBG, OGTT for 30 minutes and 120 minutes had no significant difference. There was no significant difference of the blood sugar level between the groups. The area under the curve （AUC） was reduced. The number and the size of the mononuclear cells within islet decreased when compared with D-gal group. MDA content decreased, and T-AOC activity increased significantly.Conclusion D-gal can induce structural and functional injury of the pancreas. ASP can antagonise D-gal to reduce the damage to the pancreas. The me
作者
董照瀛
黄杰
许梦雄
陈雄斌
汪子铃
肖名贺
陈粼波
姜蓉
王璐
王亚平
DONG Zhao-ying;HUANG Jie;XU Meng-xiong;CHEN Xiong-bin;WANG Zi-ling XIAO Ming-he;CHEN lin-bo;JIANG Rong;WANG Lu;WANG Ya-ping(Laboratory of Stem Cells and Tissue Engineering,Department of Histology and Embryology Chongqing Medical University,Chongqing 400016,China)
出处
《解剖学报》
CAS
CSCD
北大核心
2018年第6期740-744,共5页
Acta Anatomica Sinica
基金
国家自然科学基金(81173398)
重庆市科委基础与前沿研究项目(CStc2014jCyjA10001)
关键词
当归多糖
D-半乳糖
胰腺
损伤
HE染色
衰老小鼠
Angelica sinensis polysaccharide
D-galactose
Pancreas
Injury
HE staining
Aging mouse
