摘要
目的探讨-20℃冻存条件下分装冻融对SD大鼠血清生化检测结果的影响。方法仪器经校准、质控合格后,采集40只空白雌性SD大鼠血液,2～8℃静止30 min后离心,血清分装并用封口膜封口,每只动物分装4份。室温4 h的血清检测分析结果作为基准参考水平,其余3份血清放置于-20℃冷冻保存,分别于2 d、7 d、14 d复融后检测,比较室温4 h与-20℃冷冻保存各保存时间对生化检测结果的影响。结果-20℃冻存条件下分装冻融14 d血清生化检测结果与室温4 h比较TP、ALB、GLU、CREA可见统计学差异(P<0.05);分装冻融7 d血清生化检测结果与室温4 h比较ALT、TP、ALB、GLU、UREA、CREA、TC可见统计学差异(P<0.05);分装冻融2 d血清生化检测结果与室温4 h比较ALT、TP、ALB、GLU、CREA可见统计学差异(P<0.05);分装冻融2 d、7 d、14 d血清生化检测结果与室温4 h比较AST、TG均未见统计学差异(P>0.05)。结论-20℃分装冻融2 d、7 d、14 d血清生化检测结果与室温4 h比较,AST、TG检测结果稳定未见影响,其余七项检测指标均可见明显影响,本实验可知如无特殊情况尽量减少血清分装冻融的次数,建议最好当天检测,否则会影响检测结果的准确性。
Objective To investigate the effect of sub packaged freezing and thawing on serum biochemical test in SD rats at-20 ℃ for cryopreservation. Method After the instrument was calibrated and quality control was passed, the blood of 40 blank female SD rats was collected, stored for 30 min at 2～8 ℃, then centrifuged, the serum was dispensed and sealed with a sealing film, and each animal was dispensed in 4 portions. Serum detection analysis was done under room temperature for 4 h and the result was the reference level. The remaining 3 sera were stored at-20 ℃ for cry preserved and were re-thawed and detected after 2 d,7 d,14 d respectively. The effects of biochemical test result at room temperature for 4 h were compared with those at-20 ℃ for cryopreservation for different time. Result There were statistical differences of the concentration of TP, ALB, GLU and CREA in the serum biochemical test result between the condition of freezing-thawing at-20 ℃ for 14 d and room temperature for 4 h（P〈0.05）. There were statistical differences of the concentration of ALT, TP, ALB, GLU, UREA, CREA and TC in the serum biochemical test result between the condition of freezing-thawing at-20 ℃ for 7 d and room temperature for 4 h（P〈0.05）. There were statistical differences of the concentration of ALT, TP, ALB, GLU and CREA in the serum biochemical test result between the condition of freezing-thawing at-20 ℃ for 2 d and room temperature for 4 h（P〈0.05）.There was no statistical difference of the concentration of AST and TG in the serum biochemical test result between the condition of freezing-thawing at-20 ℃ for 2 d, 7 d, 14 d and room temperature for 4 h（P〉0.05）. Conclusion The serum biochemical test result of AST and TG were stable and consistent between the condition of freezing and thawing at-20 ℃ for 2 d, 7 d, 14 d and room temperature for 4 h while the other seven result have changed. This experiment shows that minimize the number of serum freeze-thawing as few as possible if there is no
作者
孙昌华
刘娜
王春芳
张娟
祝清芬
SUN Changhua;LIU Na;WANG Chunfang;ZHANG Juan;ZHU Qingfen(Shandong Institute for Food and Drug Control,Jinan 250101,China)
出处
《实验动物科学》
2018年第5期52-55,共4页
Laboratory Animal Science
关键词
分装冻融
SD大鼠
血清
sub packaged freezing-thawing
SD rats
serum
