摘要
目的探讨银杏内酯B(GB)预处理给药对心肌缺血/再灌注损伤大鼠能量代谢的影响。方法将大鼠随机分为5组(n=10):假手术组、模型组、GB各剂量组(8、4、2 mg/kg体质量),通过结扎冠脉30 min再灌注2 h建立心肌缺血/再灌注损伤模型,各组于术前1 h和再灌注即刻分别尾静脉注射给予1/2量的药物;再灌注结束后,采用比色法测定血清肌酸激酶(CK)和乳酸脱氢酶(LDH)活性,采用定磷法测定心肌组织Na^+/K^+-ATP酶和Ca^(2+)/Mg^(2+)-ATP酶的活力,采用染色法测定心肌梗死面积(MIS)。结果 GB高剂量组MIS缩小至16.20%,与模型组MIS(20.12%)比较差异有统计学意义(P<0.05);GB各剂量组CK活性分别降低为0.69、0.64、0.63 U/mL,与模型组(0.77 U/mL)比较差异均有统计学意义(P<0.05或P<0.01);高剂量组LDH活性降低为2.20 U·mL^(-1),与模型组(2.99 U/mL)比较差异有统计学意义(P<0.01);GB高、中剂量组可显著升高Na^+/K^+-ATP酶活力为3.69、3.78 mmol Pi/g,与模型组(3.09 mmol Pi/g)比较差异均有统计学意义(P<0.05);高、中、低剂量组可显著升高Ca^(2+)/Mg^(2+)-ATP酶活力为4.50、4.79、4.81 mmol Pi/g,与模型组(3.97 mmol Pi/g)比较差异均有统计学意义(P<0.05或P<0.01)。结论 GB预处理对心肌缺血/再灌注所致心肌损伤的保护作用可能与改善心肌组织的能量代谢有关。
Objective To investigate the effects of ginkgolide B on energy metabolism in rats with myocardial ischemia/reperfusion injury. Method SD rats were divided randomly into sham group, model group, ginkgolide B low dose group（2 mg/kg）, ginkgolide B medium dose group（4 mg/kg） and ginkgolide B high dose group（8 mg/kg）（n=10）. Myocardial ischemia/reperfusion injury model was established by coronary artery ligation（30 min） and subsequent reperfusion（2 hours） in rats. Ginkgolide B was injected via tail vein twice at 1 h pre-operation and the start of the reperfusion, respectively. After the end of reperfusion, the CK and LDH activities in sera were examined by chromatometry, Na^＋/K^＋-ATPase and Ca^2＋/Mg^2＋-ATPase activities in myocardial tissue were detected by phosphorus method and the myocardial infarction size（MIS） was determined by staining method. Result High dose of ginkgolide B significantly reduced MIS to 16.20%, comparing to 20.12% of model group（P〈0.05）. The CK activities in ginkgolide B groups were 0.69, 0.64 and 0.63 U/mL, respectively, which were significantly lower than 0.77 U/mL of model group（P〈0.05 or P〈0.01）. Ginkgolide B（8 mg/kg） significantly reduced LDH activities to 2.20 U/mL, comparing to 2.99 U/mL of model group（P〈0.01）. Ginkgolide B（8 and 4 mg/kg） evidently increased Na^＋/K^＋-ATPase activity to 3.69 and 3.78 mmol Pi/g, respectively, comparing to 3.09 mmol Pi/g of model group（P〈0.05）. Ginkgolide B significantly increased Ca^2＋/Mg^2＋-ATPase activity to 4.50, 4.79 and 4.81 mmol Pi/g, respectively, comparing to 3.97 mmol Pi/g of model group（P〈0.05 or P〈0.01）. Conclusion The protective effects of ginkgolide B pretreatment in myocardial ischemia/reperfusion injury appears to be associated with the improvement of energy metabolism.
作者
周春霞
李霞
树俊莲
徐丽
ZHOU Chunxia;LI Xia;SHU Junlian;XU Li(Department of Integrated Chinese and Western Medicine,Former Armed Police General Hospital,Beijing 100039,China;Department of Pharmacy,General Hospital of Armed Police Forces,Beijing 100039,China)
出处
《实验动物科学》
2018年第5期7-10,共4页
Laboratory Animal Science
关键词
银杏内酯B
心肌缺血/再灌注损伤
能量代谢
Ginkgolide B
myocardial ischemia/reperfusion injury
energy metabolism
