摘要
以黄灯笼辣椒‘热辣2号’及抗、感病(黄瓜花叶病毒, CMV)黄灯笼辣椒自交系为实验材料,根据已发表的辣椒基因组数据设计引物,利用RT-PCR技术从‘热辣2号’中获得了一个茉莉酸氨基合酶基因(CcJAR1)。该基因开放读码框为1 728 bp,推断其编码575个氨基酸,分子量约为64.28 kD,根据序列比对结果将其蛋白命名为CcJAR1,对其进行信息学分析表明:CcJAR1与辣椒、番茄、茄子等茄科植物的JAR1相似性较高,而与核桃、猕猴桃等其他植物的相似性较低。荧光定量PCR分析表明,该基因在‘热辣2号’黄灯笼辣椒的根中表达量最高,在叶中次之,茎中的含量最低。该基因在抗病与感病种质叶片中均响应CMV侵染的胁迫,在感染初期上调,后期下调,抗病种质中的表达量低于感病种质。CcJAR1基因是茉莉酸氨基合酶基因,在植物响应胁迫的过程中发挥着重要作用,该基因的克隆与分析将为研究黄灯笼辣椒抗病分子机制提供理论依据。
In this study, taking Capsicum chinense ‘Rela No.2＇, resistant and susceptible （CMV） selfing lines as experimental materials, a JA-amino acid synthetase gene was obtained by using RT-PCR technology. The open reading frame of the gene is 1 728 bp, which is deduced to encode 575 amino acids and the molecular weight is about 64.28 kD. According to the sequence alignment, we named the protein CcJAR1. The bioinformation analysis shows that CcJAR1 is similar to the JAR1 of the plants of pepper, tomato, eggplant and other plants of Solanaceae, but compared with other plants such as walnut and kiwi fruit, the similarity is low. Fluorescence quantitative PCR analysis shows that the expression level of the gene was the highest in the root of‘Rela No.2＇ C. chinense, lower in the leaves, and the lowest in the stem. The gene was responsive to CMV infection in leaves of resistant and susceptible C. chinense selfing lines, up regulation in the early stage of infection and down regulation in the later stage, and the expression level in the disease resistant selfing lines was lower than that of the susceptible selfing lines.Jasmonic acid is an endogenous growth regulator in plants and it is generally believed that the content rises when plants are stressed by pathogens. The CcJAR1 gene is a JA-amino acid synthetase gene and plays an important role in plant response to stress. The cloning and analysis of CcJAR1 gene will provide a theoretical basis for the study of molecular mechanism and resistant breeding of C. chinense.
作者
杨丽佳
陈欢欢
申龙斌
曹振木
曾迪
Yang Lijia;Chen Huanhuan;Shen Longbin;Cao Zhenmu;Zeng Di(Institute of Tropical Agriculture and Forestry,Hainan University,Danzhou,571737;Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture,Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,571737)
出处
《分子植物育种》
CAS
CSCD
北大核心
2018年第22期7241-7247,共7页
Molecular Plant Breeding
基金
本研究由海南省重点研发计划项目(ZDYF2017173)
中国热带农业科学院基本科研业务费专项(1630032017026)
中国热带农业科学院非营利性科研机构改革启动经费项目(pzsfyl-201819)共同资助
