摘要
目的探讨轻度认知功能障碍（MCI）的RA患者血浆中S100B蛋白、过度磷酸化tau（P-tau）蛋白、β淀粉样蛋白（Aβ1-42）浓度，为临床诊断及预防RA患者MCI提供参考。方法将研究对象分为MCI的RA患者组、认知功能正常（NC）的RA患者组和健康对照组，采用蒙特利尔认知评估量表（MoCA）评估患者的认知功能，日常生活能力量表（ADL）评估患者的日常生活能力，神经精神量表（NPI）排除痴呆，广泛性焦虑障碍量表-7（GAD-7），9条目患者健康问卷-9（PHQ-9）排除焦虑抑郁。分析3组受试者基本临床资料的差异。ELISA法检测各组血浆中S100B、P-tau、Aβ1-42蛋白的浓度，并进行Pearson相关分析。结果①认知评分结果显示，RA患者存在MCI；② ELISA结果显示，MCI的RA患者组血浆中S100B （F=11.81，P〈0.05）、P-tau （F=3.3，P〈0.05）蛋白浓度高于NC的RA患者组和对照组，差异具有统计学意义（P〈0.05）；③临床指标分析结果显示，MCI的RA患者组CRP浓度高于NC的RA患者组和健康对照组，差异具有统计学意义（t=6.44，P〈0.05）；④ 3组的血浆P-tau蛋白（r=-0.539，P〈0.05）、S100B蛋白（r=-0.346，P〈0.05）、CRP（r=-0.358，P〈0.05）浓度与认知评分呈负相关（P〈0.05）。结论CRP、S100B蛋白、P-tau蛋白与MCI的RA患者认知水平呈负相关，其血浆浓度检测结果可与认知评估问卷相结合，为临床诊断RA患者的MCI提供参考。
Objective To investigate the plasma protein concentration of S100B protein, hyperphos-phorylated tau protein （P-tau） and β-amyloid （Aβ1-42） of rheumatoid arthritis （RA） patients with mild co- gnitive impairment （MCI） and to provide a reference for clinical diagnosis and prevention of cognitive dysfunction of RA patients.MethodsThe subjects were consisted of three groups： RA patients with MCI, RA patients with normal cognitive function （NC） and healthy controls. The Montreal Cognitive Assessment （MoCA） was used to test patients＇ cognitive function, generalized anxiety disorder scale-7 （GAD-7） scale and 9-item patient health questionnaire-9 （PHQ-9） were used to exclude anxiety and depression of RA patients. The concentration of S100B protein, P-tau protein and Aβ1-42 protein in plasma was detected by enzyme-linked immunosorbent assay （ELISA）, and the correlation among the concentration of S100B protein, P-tau protein and MoCA scores was analyzed by Pearson＇s chi-squared test.Results① Cognitive scores showed that some RA patients had MCI. ② The plasma levels of S100B （F=11.81, P〈0.05）, P-tau （F=3.3, P〈0.05） protein in RA patients with MCI were higher than that in NC RA patients and the control group （P〈0.05）. ③ The clinical index analysis showed that the concentration of C reactive protein （CRP） in RA patients with MCI was higher than that in NC RA patients and healthy control, the difference was statistically significant （t=6.44, P〈0.05）. ④ The levels of plasma P-tau （r=-0.539, P〈0.05）, S100B （r=-0.346, P〈0.05）, CRP （r=-0.358, P〈0.05） protein were negatively correlated with cognitive scores （P〈0.05）.ConclusionCRP, S100B protein and P-tau protein are associated with the pathogenesis of RA patients with MCI. The consequences of plasma concentration test can be com-bined with cognitive assessment questionnaire to provide reference for clinical diagnosis of RA patients with MCI.
作者
杨莉
邹庆华
张艳
石颖
胡春蓉
惠彩霞
刘晓飞
方勇飞
Yang Li;Zou Qinghua;Zhang Yah;Shi Yin;Hu Chunrong;Hui Caixia;Liu Xiaofei;Fang Yongfei(Department of lntegrated Chinese and Western Medicine,Army Medical University,Chongqing,400038,China)
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2018年第10期649-653,共5页
Chinese Journal of Rheumatology
基金
国家自然科学基金（面上项目）（81373180）
重庆市卫生局中医药科技项目（ZY20132126）
