摘要
在宁夏枸杞(Lycium barbarum L.)品种宁杞1号花药发育差异蛋白质组学研究的基础上,克隆了一个花药发育相关基因Lb14-3-3b,证实该基因在花器官中优势表达。本试验进一步利用荧光定量PCR技术分析枸杞Lb14-3-3b基因在花药发育过程中不同时期的表达特征,并通过构建植物过表达载体Lb14-3-3b-pCambia1305. 1-35s,经根瘤农杆菌介导法转化模式植物烟草,探究该基因在植物生长发育中的功能。结果表明,在枸杞花药发育的各个时期,Lb14-3-3b都有表达,在花药二核花粉时期表达量最高。与野生型烟草相比,转Lb14-3-3b基因烟草生长发育迟缓,植株矮小,花器官畸变,花瓣缺少致使花型趋于四角化,雄蕊及花丝数目缺少且发育异常。推测枸杞Lb14-3-3b基因在烟草生长发育及花器官发育中起调控作用,研究结果为进一步探讨该基因在枸杞生长发育中的调控作用提供参考依据。
By analyzing the proteome datasets during the anther development in wolfberry variety ＇Ning Qi No.1＇,we identified and isolated an anther development related gene Lb14-3-3b that is observed in floral organs. In order to further understand its transcriptional characteristics in floral organs, we determined by real-time quantitative PCR approach the transcriptional level of Lb14-3-3b at different stages of wolfberry anther development. In addi- tion, we transformed this gene into model plant tobacco via Agrobacterium tumefaciens mediated transformation, driv- en by the constitutive 35S promoter. The results showed that Lb14-3-3b expressed at all stages of the wolfberry an- ther development, particularly at the stage of two-nuclear pollen grain with the highest expression level. Compared with wild type, transgenic tobacco plants grew delayed growth and dwarfed phenotype, floral organ distortion, such as one petals and stamen absent, four angle flower patterns. We speculate that Lb14-3-3b gene might play a role in regulation of tobacco plant growth and floral organ development, despite that its functional mechanism in wolfberry remained unclearly. Thus this work provided a starting point to further explore its functional mechanism involved in the growth and development of Lycium barbarum L..
作者
徐蕊
周丽
李瑞国
冯嘉馨
岳思君
毛桂莲
郑蕊
XU Rui;ZHOU Li;LI Rui-guo;FENG Jia-xin;YUE Si-jun;MAO Gui-lian;ZHENG Rui(Western Biological Resources Protection and Utilization Lab of National Education Ministry/ College of Life Science,Ningxia University,Yinchuan 750021)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2018年第6期1180-1186,共7页
Journal of Plant Genetic Resources
基金
国家自然科学基金(31560418)
国家自然科学基金(31360361)
国家自然科学基金(31360025)
