摘要
目的探讨PARD3B、LOC729993、EPHA4、HNT基因多态性与湘西地区侗族人群2型糖尿病(T2DM)的相关性。方法按照WHO 1999年2型糖尿病(T2DM)诊断标准,将研究对象分为T2DM患者、糖调节受损者(IGR)和正常糖耐量者(NGT),通过问卷调查、体格测量获得人口统计学资料,同时抽取血样用于生化指标检测,采用多重PCR-SNaPshot基因分型技术,对PARD3B、LOC729993、EPHA4、HNT基因的4个多态位点rs849230、rs149228、rs16862811、rs3099797进行基因分型,分析各因素与2型糖尿病的相关性。结果结果发现年龄、胰岛素抵抗指数(INSIR)、β细胞功能指数(INSβC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、体质量指数(BMI)、腰臀比(WHR)、糖尿病家族史、rs849230的AA基因型及其风险等位基因A与糖尿病和糖调节受损有关(均P<0.05);调整年龄和BMI后,rs849230位点的AA基因型与T2DM相关(OR=2.557,95%CI:1.039~6.303,P<0.05)。结论PARD3B基因的rs849230基因位点是湘西地区侗族T2DM的风险基因,可能通过影响胰岛β细胞功能引起糖尿病。
Objective To explore the association of the polymorphisms of PARD3 B gene,LOC729993 gene,EPHA4 and HNTgene with type 2 diabetes（T2 DM）among residents with Dong nationality in Western Hunan. Methods According to the WHO 1999 diagnostic criteria of type 2 diabetes mellitus（T2 DM）,study subjects were divided into three groups including patients with T2 DM,subjects with impaired glucose regulation（IGR）and subjects with normal glucose tolerance（NGT）.A questionnaire survey was conducted and physical examination was performed for each subject to collect demographic and clinical data and blood specimens were also collected and tested for biochemistry index as well as for the identification of genotypes of the rs849230,rs149228,rs16862811 and rs3099797 in PARD3 B,LOC729993,EPHA4,HNTgenes respectively by the multiplex PCR-SNaPshot method. Results Data analysis showed that age,HOMA-INSIR,HOMAINSβ,TG,HDL-C,BMI,WHR,family history of diabetes,the AA genotype and the risk alleles A of rs849230 were associated with T2 DM and IGR（P〈0.05）.AA genotype of rs849230 was associated with T2 DM after adjusting age and BMI（OR=2.557,95%CI：1.039-6.303,P〈0.05）. Conclusions The locus of rs849230 in PARD3 Bgene is a risk gene for T2 DM,and might cause diabetes by affecting the function of the INSβcell.
作者
刘立亚
陈立章
张英
田玉梅
樊婵
易宗娓
LIU Li-ya;CHEN Li-zhang;ZHANG Ying;TIAN Yu mei;FAN Chan;YI Zong-wei(School of Laboratory Medicine, Hunan Univesity of Medicine, Huaihua, Hunan 418000, China)
出处
《中国预防医学杂志》
CAS
CSCD
2018年第3期228-233,共6页
Chinese Preventive Medicine
基金
教育部高等学校博士学科点专项科研基金(博导类)资助项目(20120162110071)
湖南省自然科学基金资助项目(2017JJ2199)
湖南省教育厅科研课题(17C1138)
