摘要
通过酶联免疫吸附测定(enzyme linked immunosorbent assay,ELISA)和反转录-套式聚合酶链式反应(reverse transcription nested polymerase chain reaction,RT-nPCR)对喜食生食的云南省大理地区普通人群进行以抗体和核酸检测为主的戊型肝炎流行病学调查。结果发现,HEV IgG抗体阳性率为62.50%(65/104),IgM抗体阳性率为3.84%(4/104);HEV RNA阳性率为12.50%(13/104)。对HEV RNA阳性样本经克隆测序后进行同源性比对和进化分析,结果表明在云南大理地区流行的HEV毒株属于HEV 4型,4h亚型,且与大理羊HEV存在较高的同源性。上述发现为进一步探究我国西南地区戊型肝炎病毒的感染情况及HEV跨种间传播提供了依据。
Enzyme linked immunosorbent assay（ELISA）and reverse transcription nested polymerase chain reaction（RT-nPCR）were employed to study the epidemiology of human hepatitis E in Dali City of Yunnan Province.The results showed that the positive rates of HEV IgG and IgM were 62.50%（65/104）and3.84%（4/104）,respectively.Besides,the positive rate of HEV RNA was 12.50%（13/104）.The homology alignment and phylogenetic analysis results showed that the human HEV strains from Dali City belong to HEV 4 genotype and 4 hsubtype,which had a very close evolutionary relationship with Dali goat HEV.Findings of the current study may provide a theoretical insight into HEV infection and interspecies transmission in southwest China.
作者
李饴
王珏
郝先辉
司徒健文
赵勇琴
黄芬
华修国
LI Yi;WANG Jue;HAO Xian-hui;SITU Jian-wen;ZHAO Yongqin;HUANG Fen;HUA Xiu-guo(School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China;Medical Faculty,Kunming University of Science and Technology,Kunming 650504,China)
出处
《上海交通大学学报（农业科学版）》
2018年第5期46-50,共5页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
国家重点研究发展项目(2017YFC12OO2O3)
国家自然科学基金(31572525)
国家自然科学基金(31360619
81660338)
云南省自然科学基金(2013FB032)
中国博士后科学基金(2014M562672)
