摘要
建立了同步提取硬巢大须球白蚁巢中黄酮和多酚的方法,为硬巢大须球白蚁巢的资源开发和综合评价提供科学依据。利用回流提取法,通过单因素考察乙醇体积分数、料液比、温度和提取时间对硬巢大须球白蚁巢中黄酮和多酚含量的影响,并在此基础上进行正交试验。利用清除DPPH·自由基、·OH自由基活性方法,测试硬巢大须球白蚁巢提取液抗氧化活性。通过实验得出硬巢大须球白蚁巢中黄酮和多酚提取的最优工艺为乙醇溶液浓度50%,料液比为1∶40,提取温度为80℃,提取时间为2 h。在此条件下同时提取硬巢大须球白蚁巢中黄酮和多酚,工艺稳定性好、结果准确可靠、工艺简单、可操作性强。硬巢大须球白蚁巢中黄酮和多酚含量分别为11. 27、11. 19 mg/g。抗氧化活性测试表明硬巢大须球白蚁巢提取液对DPPH·的IC50为0. 75 mg/mL,对·OH的IC50为2. 66 mg/mL。结果表明水浴-回流法能够同步提取硬巢大须球白蚁巢中黄酮和多酚类物质,白蚁巢提取液具有一定的抗氧化活性。
Compared the content of flavonoid,polyphenol in Hospitalitermes majusculus by establishing the simultaneous extraction method,which provides a scientific basis for resource exploration and comprehensive evaluation of Hospitalitermes majuscules in the study.Analyzing the factors like ethanol volume fraction through single factor investigation,solid/liquid ratio,temperature and extraction time how to affect the content of flavonoid,polyphenol in Hospitalitermes majusculus by using refluxing extraction.Then,the orthogonal experiment was carried out.The antioxidative activity of the extracts from the Hospitalitermes majusculus was tested by using the DPPH free radical and OH free radical activitymethod. The best extracting technology for flavonoid,polyphenol in Hospitalitermes majusculuas follows： ethanol volume fraction is 50%,the liquid ratio is 1 ∶40,extracting temperature is 80 ℃,extraction time is 2 h. In this condition,the extracted process stability was good,the results were accurate and reliable,and the process was simple and operable.The research shows that the flavonoids and total phenol content was 11. 27,11. 19 mg/g,DPPH·IC50（ half clearance rate） was 0. 75 mg/mL,·OH IC50 was 2. 66 mg/mL.The results show that the water bath-reflux method can simultaneously extract the flavonoids,polyphenols in the Hospitalitermes majusculus. The extracts of Hospitalitermes majusculus has certain antioxidant activity.
作者
杨新周
郭云胶
田孟华
杨子仙
马艳粉
田先娇
YANG Xin-zhou;GUO Yun-jiao;TIAN Meng-hua;YANG Zi-xian;TIAN Xian-jiao(a.Research Institute of Ethnic Minority Medicine,lb.Department of Science and Engineering,1 c.Department of Life Sciences,Dehong Teachers＇College,Dehong 678400,China;Zhaotong Tianma Research Institute,Zhaotong 657000,China)
出处
《化学试剂》
CAS
北大核心
2018年第11期1102-1107,共6页
Chemical Reagents
基金
云南省应用基础研究青年项目(2018FD099)
民族药资源化学国家民族事务委员会-教育部重点实验室开放基金项目(MZY1602)
云南省教育厅基金资助项目(2017ZZX277)
德宏州科技创新计划项目(2017-5)
德宏师范高等专科学校优秀中青年教师学术带头人培养项目(2016-2018)
关键词
硬巢大须球白蚁
黄酮
多酚
抗氧化
Hospitalitermes majusculus
flavonoids
phenolic
antioxidan
