摘要
AIM:To evaluate telomere length in sperm DNA and its correlation with oxidative stress(normal,mild,severe).METHODS:The study included infertile men(n=112)and age matched fertile controls(n=102).The average telomere length from the sperm DNA was measured using a quantitative real time PCR based assay.Seminal reactive oxygen species(ROS)and 8-Isoprostane(8-IP)levels were measured by chemiluminescence assay and ELISA respectively.RESULTS:Average sperm telomere length in infertile men and controls was 0.609±0.15 and 0.789±0.060,respectively(P<0.0001).Seminal ROS levels in infertile was higher[66.61±28.32 relative light units(RLU)/s/million sperm]than in controls(14.04±10.67RLU/s/million sperm)(P<0.0001).The 8-IP level in infertile men was significantly higher(421.55±131.29pg/mL)than in controls(275.94±48.13 pg/mL)(P<0.001).When correlated to oxidative stress,in normal range of oxidative stress(ROS,0-21.3 RLU/s/million sperm)the average telomere length in cases was 0.663±0.14,in mild oxidative stress(ROS,21.3-35 RLU/s/million sperm)it was elevated(0.684±0.12)and in severe oxidative stress(ROS>35 RLU/s/million sperm)average telomere length was decreased to 0.595±0.15.CONCLUSION:Mild oxidative stress results in lengthening of telomere length,but severe oxidative stress results in shorter telomeres.Although telomere maintenance is a complex trait,the study shows that mild oxidative stress is beneficial in telomere length maintenance and thus a delicate balance needs to be established to maximize the beneficial effects of free radicals and prevent harmful effects of supra physiological levels.Detailed molecular evaluation of telomere structure,its correlation with oxidative stress would aid in elucidating the cause of accelerated telomere length attrition.
AIM： To evaluate telomere length in sperm DNA and its correlation with oxidative stress （normal, mild, severe）.METHODS： The study included infertile men （n = 112） and age matched fertile controls （n = 102）. The average telomere length from the sperm DNA was measured using a quantitative real time PCR based assay. Seminal reactive oxygen species （ROS） and 8-Isoprostane （8-IP） levels were measured by chemiluminescence assay and ELISA respectively.RESULTS： Average sperm telomere length in infertile men and controls was 0.609 ± 0.15 and 0.789 ± 0.060, respectively （ P 〈 0.0001）. Seminal ROS levels in infertile was higher [66.61 ± 28.32 relative light units （RLU）/s/million sperm] than in controls （14.04 ± 10.67 RLU/s/million sperm） （P 〈 0.0001）. The 8-IP level in infertile men was signifcantly higher （421.55 ± 131.29 pg/mL） than in controls （275.94 ± 48.13 pg/mL） （P 〈0.001）. When correlated to oxidative stress, in normal range of oxidative stress （ROS, 0-21.3 RLU/s/million sperm） the average telomere length in cases was 0.663 ± 0.14, in mild oxidative stress （ROS, 21.3-35 RLU/s/million sperm） it was elevated （0.684 ± 0.12） and in severe oxidative stress （ROS 〉 35 RLU/s/million sperm） average telomere length was decreased to 0.595 ± 0.15.CONCLUSION： Mild oxidative stress results in len-gthening of telomere length, but severe oxidative stress results in shorter telomeres. Although telomere maintenance is a complex trait, the study shows that mild oxidative stress is beneficial in telomere length maintenance and thus a delicate balance needs to be established to maximize the beneficial effects of free radicals and prevent harmful effects of supra physio-logical levels. Detailed molecular evaluation of telomere structure, its correlation with oxidative stress would aid in elucidating the cause of accelerated telomere length attrition.
基金
Supported by Department of Biotechnology(DBT),Government of India,No.1369
