摘要
目的分析2009-2011年引起吉林省敦化市手足口病(HFMD)流行的肠道病毒71型(EV-A71) VP1编码区氨基酸位点的变异。方法对2009-2011年分离于敦化市HFMD病例的3株EV-A71分离株的VP1编码区进行逆转录-聚合酶链反应、核苷酸序列测定,使用MEGA 4.0软件和Bioedit软件分析VP1编码区基因亲缘关系及氨基酸位点的变异。结果 2009-2011年敦化市3株EV-A71均属于C4a基因亚型,并且这3株病毒在VP1编码区第22位氨基酸位点均由谷氨酰胺转变为组氨酸(Q-H),与本研究中比对的2009-2011年吉林省代表株变异一致,也与2008年安徽阜阳的分离株变异一致。结论致敦化市2009-2011年HFMD流行的EV-A71已在全省其他县市传播流行,敦化市流行株有可能是从安徽阜阳直接或辗转传播所致。
Objective To analyze the variation of amino acid site of VP1 coding region of enterovirus 71 in Dunhua city which cause hand food and mouth disease（ HFMD） in 2009-2011. Methods 3 strains of EV-A71 virus isolated from the HFMD cases were used to do the RT-PCR and sequences of VP1 genes of EV-A71 strains,and were analyzed the gene phylogenetic and variation of amino acid site by the software of Mega4.0 and bioedit and.Results All 3 EV-A71 strains were belong to C4 a subgenotype in Dunhua city in 2009-2011,and the 3 strains had the same variation site from Q to H with those Jilin strains in the 22 th amino acid site in VP1 coding region,and also had the same variation with that Fuyang strain. Conclusion The EV-A71 which caused HFMD epidemic in Dunhua city in 2009-2011 has been spreading in other counties in Jilin province,and maybe direct or indirect from continuing transmission of Fuyang strains.
作者
杨金红
魏雷雷
田丽敏
王爽
田鑫
曹凤瑞
程涛
单元春
付思美
吕淑梅
李大强
YANG Jin-hong, WEI Lei-lei, TIAN Li-min, WANG Shuang, TIAN Xin, CAO Feng-rui, CHENG Tao, SHAN Yuan-chun, FU Si-mei, LV Shu-mei, LI Da-qiang(Department of AIDS and Venereal Disease Control and Prevention, Dunhua Center for Disease Control and Prevention, Dunhua Jilin 133000, China)
出处
《中国卫生工程学》
CAS
2018年第5期690-692,共3页
Chinese Journal of Public Health Engineering
关键词
HFMD
肠道病毒71型
氨基酸位点
变异
Hand foot and mouth disease
Enterovirus type 71
Amino acid site
Variation
