摘要
目的通过痛经模型对产地鲜切加工乌药饮片与传统加工乌药饮片进行药效比较。方法采用雌激素诱导小鼠痛经模型,通过记录小鼠扭体反应及前列腺素E2(PGE2)、前列腺素F2α(PGF2α)、血管加压素(AVP)、β-内啡肽(β-EP)5个指标的变化来比较2种不同加工方式乌药治疗痛经的效果。结果与模型组比较,2种不同加工方式乌药均能明显减少痛经模型小鼠扭体次数(P<0.01),降低PGE2、PGF2α、AVP水平(P<0.05、0.01),升高β-EP水平(P<0.01),但两者相同给药剂量结果差异不显著(P>0.05)。结论两种不同加工方法乌药均对痛经模型小鼠起到良好的治疗效果,并且产地鲜切加工乌药与传统加工乌药对痛经模型小鼠的药效强度相当。
Objective To evaluate the anti-dysmenorrhea efficacy of habitat fresh-cut processed and traditional processed Linderae Radix based on the dysmenorrhea model.Methods By using the estrogen-induced dysmenorrhea model of ICR mice,the effect of treatment from two different processing methods on dysmenorrhea was compared by recording the writhing reaction as well as the changes of levels of prostaglandin E2（PGE2）,prostaglandin F2α（PGF2α）,vasopressin（AVP）,and β-endorphin（β-EP）.Results Compared with the model group,Linderae Radix from two different processing methods significantly reduced the frequency of writhing（P 0.01）,reduced the contents of PGE2,PGF2α,and AVP（P 0.05,0.01） and increased the content of β-EP（P 0.01）.However,there was no significant difference between two methods of Linderae Radix administration for the same dose（P 0.05）.Conclusion The two processing methods of Linderae Radix both have good therapeutic effect on dysmenorrhea model mice,and the effect of habitat processed Linderae Radix is not inferior to that of traditional processed Linderae Radix.
作者
罗云云
康显杰
杨莹
张婷
朱涛
吴瑶
杜伟锋
葛卫红
LUO Yun-yun;KANG Xian-jiel;YANG Ying;ZHANG Ting;ZHU Zao;WU Yao;DU Wei-feng;GE Wei-hong(College of Pharmaceutical Science,Zhejiang Chinese Medical University,Hangzhou 310053,China;Research Center of Traditional Chinese Medicine Processing Technology,Zhejiang Chinese Medical University,Hangzhou 311401,China)
出处
《中草药》
CAS
CSCD
北大核心
2018年第20期4731-4736,共6页
Chinese Traditional and Herbal Drugs
基金
国家中药标准化项目(ZYBZH-H-ZY-45)
中医药行业科研专项"中药饮片质量保障系统研究(一)"(201507002)
浙江省公益性技术应用研究计划资助项目(2015C33152)
浙江中医药大学重中之重一级学科开放基金项目(2016)
国家自然科学基金预研专项(2016ZG19)
