摘要
目的探讨RNA解旋酶DDX3与转移性乳腺癌预后的关系。方法收集2010年1月至2012年12月间就诊金华市中心医院确诊为转移性乳腺癌(Ⅳ期)的患者共83例,经RT-PCR技术检测术后癌组织及癌旁组织RNA解旋酶DDX3的表达情况,分析其与转移性乳腺癌患者术后生存状况的关系。结果通过RT-PCR检测结果显示,RNA解旋酶DDX3在转移性乳腺癌组织中的表达(0.49±0.06)显著高于癌旁组织的表达量(0.23±0.02)(t=4.956,P<0.05)。同时,RNA解旋酶DDX3在转移部位、转移部位数、雌激素受体/孕激素受体(ER/PR)状态临床因素中表达存在明显差异(t/F值分别为8.013、9.227、8.246,均P<0.05),而在年龄、是否绝经、癌组织类型临床因素中不存在明显差异(t/F值分别为1.432、0.914、1.107,均P>0.05)。DDX3的表达水平≥0.45在术后3年的复发率明显高于其表达水平<0.45者(χ2=8.121,P<0.05)。同时,DDX3的表达水平≥0.45者的平均复发时间也短于其表达水平<0.45者,两组差异均具有统计学意义(t=7.235,P<0.05)。利用Cox风险回归模型分析结果显示,转移部位、转移部位数、DDX3的表达量是转移性乳腺癌术后预后的独立危险因素(OR值分别为1.427、1.203、2.886,均P<0.05),术后辅助放化疗是保护因素(OR=0.779,P<0.05)。结论 RNA解旋酶DDX3是转移性乳腺癌患者术后预后的影响因素,通过对RNA解旋酶DDX3的检测可为转移性乳腺癌患者临床诊治提供一定的参考价值。
Objective To investigate the relationship between RNA helicase DDX3 and prognosis of metastatic breast cancer（MBC）.Methods From January 2010 to December 2012,83 cases of MBC（stage Ⅳ）were diagnosed in Jinhua Center Hospital.The expression of RNA helicase DDX3 in cancer tissues and paracancerous tissues was detected by reverse transcription-polymerase chain reaction（RT-PCR）technique to analyze its correlation with postoperative survival in patients with MBC.Results RT-PCR detection revealed that the expression of RNA helicase DDX3 in cancer tissues（0.49±0.06）was significantly higher than in adjacent tissues（0.23±0.02）（t=4.956,P〈0.05）.The expression of RNA helicase DDX3 in location of metastasis,number of metastatic site and ER/PR status was significantly different（t/F value was 8.013,9.227 and 8.246,respectively,all P〈0.05）,but there was no significant difference in age,menopause and cancer type（t/Fvalue was 1.432,0.914 and 1.107,respectively,all P〉0.05）.The recurrence rate of the cases with DDX3 expression≥0.45 three years after operation was significantly higher than that with DDX3 expression0.45（χ^2=8.121,P〈0.05）.Meanwhile,the average recurrence time of the cases with DDX3〉0.45 was shorter than that with DDX30.45,and the difference between two groups was statistically significant（t=7.235,P〈0.05）.Cox risk regression analysis showed that the location of metastasis,the number of metastasis site and the expression of DDX3 were independent risk factors for prognosis of MBC（OR value was 1.427,1.203 and 2.886,respectively,all P〈0.05）.Postoperative adjuvant radiotherapy and chemotherapy were protective factors（OR=0.779,P〈0.05）.Conclusion RNA helicase DDX3 is an important influencing factor for postoperative prognosis of patients with MBC.The detection of RNA helicase DDX3 can provide reference for clinical diagnosis and treatment of MBC.
作者
胡金飞
胡望远
HU Jin fei;HU Wang-yuan(Department of Radiotherapy,Jinhua Center Hospital,Zhej iang Jinhua 321000,China)
出处
《中国妇幼健康研究》
2018年第10期1264-1268,共5页
Chinese Journal of Woman and Child Health Research
