摘要
目的 观察分析性别决定基因盒9 （sex-determining region Y box 9,SOX9）的表达水平对口腔鳞状细胞癌（oral squamous cell carcinoma,OSCC）细胞增殖、迁移和转移的影响.方法 纳入2008年1月至2012年9月上海市口腔颌面部肿瘤组织样本及生物信息学数据库共享服务平台OSCC患者的病理组织标本74例,免疫组化法检测OSCC组织中SOX9蛋白的表达水平并分析其与临床特征的关系;细胞计数检测细胞增殖和平板克隆实验观察SOX9的表达与OSCC细胞增殖的关系;Transwell和划痕实验检测不同SOX9表达水平的OSCC细胞迁移能力.结果 高表达SOX9的OSCC患者淋巴结转移风险显著提高（P=O.010）.Transwell实验中HN6（671.0±57.4,P=0.000）细胞均较CAL27细胞（172.0±13.9）迁移到下室的细胞数多,而划痕实验中HN6[0 h：（97.3±2.1）μm;6h：（56.7±2.5）μm;12 h：（29.7±3.1）μm]较CAL27[0 h：（93.7±1.5） μm;6 h：（78.0±2.0）μm;12 h：（42.0±3.0）μm]迁移速度快（P＜0.05）.高表达SOX9的OSCC细胞系（HN6）细胞的迁移能力显著高于SOX9低表达的细胞系（CAL27）细胞（P＜0.05）且不同SOX9表达水平对OSCC细胞增殖无明显影响（P＞0.05）.结论 SOX9蛋白高表达可促进OSCC的迁移和淋巴结的转移能力,SOX9是OSCC淋巴结转移分子诊断和干预的候选分子靶点.
Objective To investigate the effects of sex-detemining region Y box9 （SOX9）expression levels on the proliferation,migration and metastasis in oral squamous cell carcinoma （OSCC）.Methods A total of 74 OSCC pathological specimens were collected from Shanghai OSCC Tissue and Biological Informations Bank,and clinicopathological information of these specimens were collected.Immunohistochemistry assay was used to examine the expression levels of SOX9 in OSCC and to analyze their relationship with clinicopathological features.Cell counting kit-8 assay and cloning formation was used to observe the relationship between the expression levels of SOX9 and the proliferation of OSCC.Transwell experiment and scratch test were used to detect the difference of the ability of OSCC in cell lines with different expression levels of SOX9.Results The risk of lymph node metastasis in patients with high expression of SOX9 was significantly increased （P=0.010）.In the Transwell experiment,the number of HN6 cells （671.0±57.4,P=0.000） migrated to the lower chamber more than that of CAL27 cells （172.0± 13.9）.In the scratch experiment,HN6 cells [0 h：（93.7±2.1） μm;6 h：（56.7±2.5） μm;12 h：（29.7±3.1） μm] migrated faster than CAL27 [0 h：（93.7±1.5） μm;6 h：（78.0±2.0） μm;12 h：（42.0±3.0） μm]（P〈0.05）.The migration ability of the cell line （HN6） with high-expression of SOX9 was significantly higher than that in cell line （CAL27） with low-expression SOX9 （P〈0.05）.The expression levels of SOX9 in OSCC were no significant on cell proliferation （P〉0.05）.Conclusions High expression of SOX9 can promote the migration and lymph node metastasis of OSCC.SOX9 is a candidate gene target for the diagnosis and intervention of lymph node metastasis in OSCC.
作者
邹忠涛
胡温庭
曹巍
陈万涛
Zou Zhongtao;Hu Wenting;Cao Wei;Chen Wantao(School of Stomatology,Weifang Medical University Weifang Shandong 261000,China;Depertment of Stomatology,Affiliated Hospital of Weifang Medical Universit;Department of Oral Maxillofacial-Head and Neck Oncology,Ninth People＇s Hospital,Shanghai Jiao Tong University School of Medicine ＆ Shanghai Key Laboratory of Stomatology ＆ Shanghai Research Institute of Stomatology ＆ National Clinical Research Center of Stomatology,Shanghai 200010,China)
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2018年第10期688-693,共6页
Chinese Journal of Stomatology
基金
国家重点研发计划（2016YFC0902703）
