摘要
采用壳聚糖为壁材、乳化交联法制备载亚硒酸钠微球。以微球形貌、包封率、载药量和缓释性能为指标,通过单因素及正交试验优化制备工艺条件,并用SEM扫描电镜、红外、原子荧光、TG热重等分析手段对微球粒径外观和结构性能进行表征。以自配的SBF模拟体液为体外环境,研究微球缓释硒的性能。结果显示,制备微球的最佳工艺条件为温度65℃,壳聚糖浓度3%,亚硒酸钠浓度0. 8%,交联剂用量15%,该条件下的微球形貌优良,包封率和载药量分别为65. 89%,5. 05%,微球平均粒径为10μm;体外缓释试验表明,通过改变相关变量因素水平,可调控球壁厚度和交联度是影响释放速率的关键因素,实现载硒微球硒的可控缓释;最佳工艺制备的微球具有较好的长效缓释能力,缓释速率在482 h后达平稳,有效缓释时间达35 d。壳聚糖载硒微球能有效避免硒的突释效应,可为缺硒群体的科学补硒提供新途径。
Chitosan was chosen as wall material to prepare the Na_2SeO_3-loading microspheres with emulsion-crosslink method. Then,the microspheres＇ appearance,drug loading capacity,encapsulation efficiency and its sustained-release property were chosen as evaluation criteria to optimize the process condition through the single factor experiments and the orthogonal tests. In addition to this,the structure of the microspheres was characterized preliminarily by the scanning electron microscopy（SEM）,infrared spectroscopy（IR）,atomic fluorescence,thermogravimetric analysis（TG）,and its sustained-release property were researched in vitro under the circumstance of the simulated body fluid（SBF） which was prepared by ourselves. The best process condition to prepare the microspheres shown by the experimental results were as follow： the temperature was 65 ℃,the concentration of chitosan and sodium selenite was 3% and 0. 8%,the dosage of crosslinking agent was 15%. Under such environment,the appearance of microspheres was pretty good,its encapsulation efficiency and drug-loading rate was 65. 89% and 5. 05% respectively and the average particle size was about 10 μm. The sustained-release rate of microspheres became stabilized after releasing for 482 h and its valid sustained-release time could last for 35 d. This experiment was expected to provide a new way to supply Se reasonably for the Se-shortage population.
作者
张驰
商龙臣
吴少魏
李光大
刘信平
ZHANG Chi1 ,SHANG Long-chen1, WU Shao-wei2,LI Guang-da3, LIU Xin-ping2(1. College of Biological Science and Technology, Hubei Minzu University, Enshi , Hubei 445000, China; 2. College of Chemistry and Environment Engineering, Hubei Minzu University, Enshi, Hubei 445000, China;3. College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, Henan 471003, China)
出处
《食品与机械》
CSCD
北大核心
2018年第8期39-44,59,共7页
Food and Machinery
基金
国家自然科学基金资助项目(编号:21461009
81402225)
恩施州重点科技项目(编号:D20170039)
关键词
壳聚糖
亚硒酸钠
微球
制备
缓释
chitosan
selenium
microsphere
preparation
sustai-ned-release
