摘要
从分离谱图、分析时间、线性范围、检出限、精密度和加标回收率角度,对离子色谱和高效液相色谱(HPLC)测定水中的短链脂肪酸的方法进行比较。HPLC使用SunFire^(TM)C18色谱柱,流动相为磷酸二氢钾溶液和甲醇,梯度洗脱,流速1.0 m L/min。离子色谱法使用Dionex^(TM)ATC-3色谱柱,流动相为不同浓度氢氧化钠溶液梯度洗脱,流速1.5 m L/min。结果表明:HPLC的分析时间、线性范围、检出限、保留时间精密度、峰面积精密度和加标回收率分别为14.5 min、0~1 200 mg/L、15.57~23.23 mg/L、0.079%~0.597%、1.12%~3.50%和96.1%~112.0%,离子色谱法的相应指标分别为13.0 min、0~20 mg/L、0.03~0.10 mg/L、0.000%~0.053%、1.18%~2.69%和98.2%~122.0%。HPLC线性检测范围宽,但检出限相对高。离子色谱法检出限低,但线性检测范围窄,过高的进样质量浓度使得分离效果不佳。应根据样品实际质量浓度,选择合适检测方法。
The determination of short-chain fatty acid by ion chromatography and high-performance liquid chromatography（HPLC）was compared from the aspects of separation chromatography,analysis time,linearity range,limit of detection,precision and recovery.HPLC method used SunFire^（TM）C18 chromatography column,mobile phase was KH_2PO_4solution and methanol,and flow rate was 1.0 m L/min.Ion chromatography method used Dionex^（TM）ATC-3 chromatography column,mobile phase was sodium hydrate solution with different concentrations,and flow rate was 1.5 m L/min.The results showed that analysis time,linearity range,limit of detection,precisions of retention time and peak area,and recovery of HPLC method were 14.5 min,0-1 200 mg/L,15.57-23.23 mg/L,0.079%-0.597%,1.12%-3.50%and 96.1%-112.0%respectively.For ion chromatography method,they were 13.0 min,0-20 mg/L,0.03-0.10 mg/L,0.000%-0.053%,1.18%-2.69%and 98.2%-122.0%,respectively.HPLC method had wide detection range,but higher limit of detection.Ion chromatography method had lower limit of detection,but narrow detection range,and higher sampling mass concentrationled to poor separation effect.So,the proper detection method should be chosen according to the actual mass concentration of sample.
作者
孟令璐
李徐
郭伟
薛晓凡
张帆
唐天培
刘睿杰
MENG Linglu;LI Xu;GUO Wei;XUE Xiaofan;ZHANG Fan;TANG Tianpei;LIU Ruijie(School of Food Science and Technology,Jiangnan University,Wuxi 214122,Jiangsu,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2018年第10期147-149,共3页
China Oils and Fats
基金
江苏高校品牌专业建设工程资助项目(PPZY2015A052)
江南大学大学生创新训练计划资助(2017177Y)
关键词
离子色谱法
高效液相色谱法
短链脂肪酸
方法比较
ion chromatography
high- perform-ance liquid chromatography
short - chain fattyacid
method comparison
