摘要
目的探索早期婴儿肠道乳酸杆菌的优势菌种及生化特征。方法收集41名1~4月龄健康婴儿粪便,采用选择培养基分离乳酸杆菌,通过形态、好氧性、碳水化合物代谢(API 50)和16S RNA测序等分析技术对分离菌株进行分类学鉴定,以及使用API ZYM检测其19种酶活性。结果共收集71株乳酸杆菌,包括副干酪乳杆菌(L.paracasei)30株,格氏乳杆菌(L.gasseri)13株,唾液乳杆菌(L.salivarius)10株,短乳杆菌(L.brevis)4株,鼠李糖乳杆菌(L.rhamnosus)3株,发酵乳杆菌(L.fermentum)和植物乳杆菌(L.plantarum)各1株,未鉴定到种9株。7种乳酸杆菌对49种碳水化合物代谢有异同,有14种碳水化合物均不能发酵,均能发酵的碳水化合物有4种,在其余31种碳水化合物的代谢上具有菌种差异。大多数乳酸杆菌的亮氨酸芳胺酶、半乳糖苷酶和葡萄糖苷酶活性强,其他酶活性因种株不同差异较大。结论副干酪乳杆菌、格氏乳杆菌和唾液乳杆菌可能是1~4月龄健康婴儿肠道乳酸杆菌的主要菌种。婴儿肠道乳酸杆菌的碳水化合物利用能力和酶活性具有一定的共同特征和种株差异。
Objective To collect intestinal lactobacilli in healthy infants and to characterize them bioehemically for further studies. Methods Lactobacilli were isolated from the fecal samples collected from total 41 of 1 - 4 month old healthy infants with culture method by selective medium. After morphological observation and oxygen test, the isolates were tested for their abilities to use 50 carbohydrate and identified with API 50 CHL system and 16S RNA sequencing. The isolates were also detected for their 19 enzyme activities with API ZYM system. Results A total of 71 Lactobacillus strains were successfully isolated and well identified, including L. paracasei （42.3%） , L. gasseri （18.3%）, L. salivarius （14.1%）, L. brevis （5.6%）, L. rhamnosus （4. 2%）, L.fermentum（ 1.4% ） , L. plantarum （ 1.4% ） and other species （ 12.7% ）. The seven species of Lactobacillus had strong ability of utilizing carbohydrate. Except for 14 carbohydrates that could not be fermentable, 4 kinds of carbohydrates could be metabolized by all lactobacilli from the seven species, and the other 31 kinds of carbohydrates were metabolized differently among species. Most lactobacilli were active on Leucine arylamidase, Galactosidase and Glucosidase, while other enzyme activities were species-specific and strains-specific. Conclusion L. paracasei, L. gasseri and L. salivarius could be considered as dominant species of 1 -4 months healthy infants. However, the carbohydrate utilization and enzyme activities of the fecal lactobacilli might be species and strain dependent.
作者
李姗珊
万群
程如越
葛林
伍梓汐
李鸣
张旭光
肖健
黄远英
何方
Li Shanshan;Wan Qun;Cheng Ruyue;Ge Lin;Wu Zixi;Li Ming;Zhang Xuguang;Xiao Jian;Huang Yuanying;He Fang(Department of Nutrition,Food Safety and Toxicology,West China School of Public Health,Sichuan University,Chengdu 610041,China)
出处
《卫生研究》
CAS
CSCD
北大核心
2018年第5期776-781,共6页
Journal of Hygiene Research
基金
国家自然科学基金(No.81372982)
关键词
乳酸杆菌
婴儿
碳水化合物
酶活性
lactobacilli
infant
carbohydrate
enzyme activity
