摘要
为探索生长环境对克氏原螯虾(Procambarus clarkii)细菌群落多样性的影响,本实验利用Illumina MiSeq测序技术,对克氏原螯虾尾肉、养殖水体及周围土壤进行基因序列测序,分析小龙虾虾肉与其生长环境之间的相关性。多样性分析结果表明,土壤的微生物多样性高于小龙虾和水体多样性,分析三组样品在不同分类水平的相对丰度发现,小龙虾、土壤和水体中有相对固定的优势菌群,在门类为变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes),α-变形菌纲(Alphaproteobacteria)、β-变形菌纲(Betaproteobacteria)和δ-变形菌纲(Deltaproteobacteria)等在小龙虾尾肉、土壤及水体中的平均相对丰度较高,而在科和属水平上无共有优势菌群。PICRUSt2代谢功能预测结果表明,3组样品在有氧呼吸(aerobic respiration I)、脂肪酸合成(fatty acid elongation)、氨基酸生物合成(amino acid synthesis)和核糖核苷酸代谢途径(nucleotide sugar metabolism)中代谢通路丰度最高。研究结果进一步解释了生长环境和克氏原螯虾微生物群落结构间的相关性和差异性。
In order to explore the effects of growth environment on the bacterial community diversity of Procambarus clarkia,we used Illumina miseq sequencing technology to sequence the tail meat of Procambarus clarkii,the culture water and the soil around the culture environment,and analyzed the correlation between the meat and the growth environment of Procambarus clarkii.The results showed that the diversity of Proteobacteria,Bacteria and Proteobacteria were higher than that of Proteobacteria and Bacteria in water.The average relative abundance of Deta Proteobacteria and Delta Proteobacteria were higher in crayfish tail meat,soil and water,but there was no common dominant flora at the family and genus level.The results of PICRUSt2 metabolic function prediction showed that the metabolic pathway abundance of three groups of samples was the highest in aerobic respiration(I),fatty acid synthesis(fat acid synthesis),amino acid biosynthesis(amino acid synthesis)and nucleoside sugar metabolism pathway(nucleotide sugar metabolism).The results of the study further explained the correlation and difference between the growth environment and the microbial community structure of Procambarus clarkia.
作者
李雪红
李述刚
石钢鹏
罗小莹
康峻
苏静
汪兰
LI Xuehong;LI Shugang;SHI Gangpeng;LUO Xiaoying;KANG Jun;SU Jing;WANG Lan(Agricultural Products Processing Research Sub-center of Hubei Agricultural Science and Technology Innovation Center,Institute for farm Products Processing and Nuclear-Agricultural Technology,Hubei Academy of Agricultural Science,Wuhan 430064,China;School of Food and Biological Engineering,Hubei University of Technology,Wuhan 430064,China;Hubei Qianwang Ecological Crayfish Industrial Park Group Corporation,Qianjiang 433100,China)
出处
《食品工业科技》
CAS
北大核心
2021年第16期91-98,共8页
Science and Technology of Food Industry
基金
国家重点研发计划(2018YFD0901001)
湖北省技术创新专项重大项目(2019ABA087)。
关键词
克氏原螯虾
土壤
水体
菌群结构
高通量测序
Procambarus clarkii
soil
water
flora structure
high-throughput sequencing
