摘要
为探究土壤调理剂对植烟土壤微生物功能多样性的影响,以烤烟品种云烟87为材料,采用BiologECO检测法探究了土壤调理剂在CK(0 kg/hm^2)、T1(1 500 kg/hm^2)、T2(3 000 kg/hm^2)、T3(4 500 kg/hm^2)四种不同施用水平下植烟土壤微生物碳代谢指纹的差异。结果表明:(1)当土壤调理剂的施用量为3 000 kg/hm2时,烤烟成熟期羧酸类、胺类、聚合物类、其他类这4类碳源的利用能力最强。(2)主成分分析结果显示,糖类、羧酸类、氨基酸类、胺类这4类碳源是区分施用土壤调理剂的敏感碳源,而聚合物类和其他类碳源是区分土壤调理剂施用量的敏感碳源。(3)土壤微生物群落功能多样性指数结果显示,T2处理的均一性指数最高,较对照提高了82.85%,而各处理间的丰富度指数与优势度指数差异不显著。由此可见,施用土壤调理剂可显著提高植烟土壤微生物碳代谢强度、群落功能多样性,3 000 kg/hm^2的施用量表现最优。上述结果为植烟土壤中土壤调理剂的施用提供一定的理论依据。
In order to explore the effects of soil conditioner on soil microbial functional diversity in tobacco planting fields,this study took flue-cured tobacco variety Yunyan 87 as material to explore the differences of microbial carbon metabolism fingerprints in tobacco planting soil by Biolog-Eco detection method under 4 different levels of soil conditioner including CK（ 0 kg/hm^2）,T1（ 1 500 kg/hm^2）,T2（ 3 000 kg/hm^2）,T3（ 4 500 kg/hm^2）. The results showed that：（1） When the application amount of soil conditioner was 3 000 kg/hm2,flue-cured tobacco had the strongest ability to use the 4 kinds of carbon sources,including carboxylic acids,amines,polymers and other types during tobacco maturing period.（2） Principal component analysis showed that there were 4 kinds of sensitive carbon sources to distinguish soil conditioner,including carbohydrate,carboxylic acids,amino acids and amines; while sensitive carbon sources polymers and other types were to distinguish soil conditioner application amount.（3） Soil microbial community functional diversity index showed that T2 had the highest of mc Intosh index,82.85% higher than that of the CK. Moreover,there were no significant difference in Richness index and Simpson index among the 4 treatments. Thus, it could be seen that the application of soil conditioner had significantly improved carbon metabolism intensity and community functional diversity of the tobacco planting soil. 3 000 kg/hm^2 application ratehad the best performance. The above results provided certain theoretical basis for applying soil conditioner in tobacco planting fields.
作者
淡俊豪
齐绍武
黎娟
朱益
靳辉勇
梁仲哲
DAN Junhao;QI Shaowu;LI Juan;ZHU Yi;JIN Huiyong;LIANG Zhongzhe(College of Agronomy,Hunan Agricultural University,Changsha 410128;Hunan Hybrid Rice Research Center,Changsha 410128;China Tobacco Guangdong Industrial Co.,Ltd.,Guangzhou 510610;Agricultural Committee of Suyu Distriction in Suqian City of Jiangsu Province,Jiangsu Suqian 223800,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2018年第10期36-43,共8页
Journal of Agricultural Science and Technology
基金
湖南省科技计划项目(2015NK3002)资助
