摘要
[目的]研究不同品种烤烟根际微生物群落结构及多样性差异。[方法]以不同烤烟品种云烟87、云烟97、云烟99和K326为材料,在2015和2016年分别进行田间试验,通过Biolog方法比较不同烤烟品种之间根际土壤微生物群落结构及多样性差异。[结果]土壤微生物群落代谢活性表现为K326>Y97>Y87>Y99;云烟99根际微生物对羧酸类碳源利用最多,而云烟87、云烟97、K326根际微生物对碳水化合物碳源利用最多;主成分分析结果显示,碳水化合物、羧酸、氨基酸与第一主成分(PC1)相关性较高,这3类碳源是区分云烟99与其他3个品种间差异的敏感碳源;不同品种的烤烟,其根际微生物种类和优势种群数量并无差异,但微生物种群的分布情况存在显著差异,尤以K326根际微生物群落物种分布最均匀。[结论]该研究为改善烤烟根际土壤微生态提供理论依据。
[Objective] To study microbial community structure and diversity in rhizosphere of different flue-cured tobacco. [Method]Field experiments were conducted in 2015 and 2016 respectively to study the difference of rhizosphere microbial community structure and functional diversity with Biolog technique among Yunyan 87, Yunyan 97, Yunyan 99 and K326 four flue-cured tobacco cultivars. [Result]Metabolic activity of the soil microbial communities followed the order：K326〉Y97〉Y87〉Y99. Carboxylic acids was the most used carbon source by Yunyan 99,but carbonhydrates was the most used carbon source by Yunyan 87, Yunyan 97, K326. The result of principal component analysis showed that these 3 types of carbon source were carbonhydrates, carboxylic acids and amino acids, Which had a high relevance of PC1 and that were used to distinguish Yunyan 99 and other. There was no difference for different flue-cured tobaccos between microbial species and dominant population, but there was significant differences in the distribution of microorganism population. The most uniform was K326.[Conclusion]This study provides theoretical basis for improving the soil microecology of flue-cured tobacco rhizosphere.
作者
李强
孙敬钊
皮本阳
黄文华
孔午圆
陈苏
贺非
LI Qiang;SUN Jing-zhao;PI Ben-yang(Linli County Branch of Changde Municipal Tobacco Company,Changde,Hunan 415000)
出处
《安徽农业科学》
CAS
2018年第30期160-163,共4页
Journal of Anhui Agricultural Sciences
关键词
根际土壤
微生物
功能多样性
烤烟
Rhizosphere soil；Microorganism；Functional diversity；Flue-cured tobacco
