摘要
目的探索间隙连接蛋白37(CX37)在腰椎椎间盘突出症炎性反应中的意义。方法观察10例腰椎椎间盘突出症患者(观察组)及8例腰椎爆裂骨折患者(椎间盘部分损坏无明显退变,对照组)CT与MRI表现差异,采用Pfirrmann分级评估椎间盘退行性变程度。利用PCR方法检测患者椎间盘组织中CX37、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的表达,并分析CX37与IL-6、TNF-α表达的相关性。结果对照组影像学资料主要表现为不同程度的椎间盘损伤,观察组主要表现为椎间盘突出、髓核脱垂、髓核突出。对照组Pfirrmann分级Ⅰ级6例,Ⅱ级2例;观察组Ⅲ级3例,Ⅳ级3例,Ⅴ级4例。PCR结果显示观察组CX37、IL-6和TNF-α的m RNA表达水平均高于对照组,差异有统计学意义(P <0.05)。Pearson相关性分析显示,炎症因子IL-6,TNF-α的表达与CX37均呈正相关。结论腰椎椎间盘突出症患者髓核中CX37表达显著高于无椎间盘退行性变病例,且与炎症因子IL-6,TNF-α的表达均呈正相关,CX37可能通过调控炎症因子的表达参与椎间盘突出症的发生、发展。
Objective To explore the clinical significance of connexin 37（CX37） in the inflammatory response in patients with lumbar disc herniation. Methods To observe the difference in CT and MRI manifestations between 10 cases of lumbar disc herniation（observation group） and 8 cases of lumbar burst fracture（intervertebral disc partial damage without obvious degeneration,control group）. Pfirrmann grading was used to evaluate the degree of disc degeneration. The expression of CX37,interleukin-6（IL-6） and tumor necrosis factor-α（TNF-α） in the intervertebral disc tissues of patients were detected by PCR,and the correlations between CX37,IL-6 and TNF-α were analyzed. Results The imaging findings showed intervertebral disc injuries mainly in the control group,and nucleus pulposus prolapse and nucleus pulposus protrusion maily in the observation group. In the control group,there were 6 cases in Pfirrmann gradeⅠand 2 in gradeⅡ;in the observation group,there were 3 in gradeⅢ,3 in gradeⅣ and 4 in gradeⅤ. The results of PCR showed that the mRNA expression levels of CX37,IL-6 and TNF-α in the observation group were higher than those in the control group,and the differences were statistically significant（P〈0.05）. Pearson correlation analysis showed that inflammatory factors IL-6 and TNF-α were positively correlated with CX37. Conclusion The expression of CX37 in nucleus pulposus of the patients with lumbar disc herniation would be significantly higher than that of those cases without intervertebral disc degeneration,and is positively correlated with the expression of inflammatory factors IL-6 and TNF-α. CX37 may participate in the development and progression of lumbar disc herniation by regulating the expression of inflammatory factors.
作者
张琛琳
陈思
陈剑峰
ZHANG Chen-lin;CHEN Si;CHEN Jian-feng(Graduate School,Nanjing University of Chinese Medicine,Nanjing 210046,Jiangsu,China;Department of Orthopaedics,Chinese Medicine Hospital of Wuxi City,Wuxi 214000,Jiangsu,China)
出处
《脊柱外科杂志》
2018年第5期302-306,共5页
Journal of Spinal Surgery
基金
无锡市卫生局面上指令性项目(ML201317)
关键词
腰椎
椎间盘移位
连接蛋白类
白细胞介素6
肿瘤坏死因子Α
Lumbar vertebrae
Intervertebral disc displacement
Connexins Interleukin-6
Tumor necrosis factor-alpha
