摘要
以西瓜为供试材料,采用聚已二醇(PEG-6000)模拟干旱试验,设置PEG-6000浓度0,9%,17%,24%和33%共5个梯度,研究干旱胁迫对西瓜叶抗氧化酶活性、叶绿素荧光参数及根系活力的影响。结果表明,叶片H_2O_2含量和O_2^-·含量随干旱胁迫强度的增加而增加,而POD和SOD活性呈现先升高后降低的单峰变化规律,在PEG-6000浓度为24%时最大,较对照分别提高156.67%和226.90%;Fo随干旱胁迫程度的增加而增加,而Fm,Fv/Fm和qP三者变化规律类似,均随胁迫强度的增加而降低。西瓜幼苗根系活力与干旱胁迫强度呈极显著负相关,其拟合方程为y=0.6739e^(-1.5156x)。
The effects of drought stress on antioxidant enzyme activity, green chlorophyll fluorescence parameters and root activity of watermelon leaves were studied by using polydiol(PEG-6000) simulated drought test and 5 gradients of PEG-6000 concentration 0,9%, 17%, 24% and 33% with watermelon as the test materials. The results showed that the content of H_2O_2 and O_2^-·in leaves increased with the increase of drought stress, while the activity of POD and SOD increased first and then decreased, and when the concentration of PEG-6000 was 24%, the activity of POD and SOD were the maximum(156.67% and 226.90%, respectively). The initial fluorescence(Fo) increased with the increase of drought stress, while the maximum fluorescence(Fm), the maximum photochemical efficiency(Fv/Fm) and the photochemical death coefficient(qP) three were similar, and all decreased with the increase of stress intensity. The root activity of watermelon seedlings was significantly negatively correlated with drought stress intensity, and the fitting equation of drought stress intensity was y=0.6739e^(-1.5156x).
作者
张金民
任晓雪
秦伟
陈昆
ZHANG Jinmin;REN Xiaoxue;QIN Wei;CHEN Kun(Shangqiu Academy of Agriculture and Forestry Sciences,Shangqiu,Henan 476000,China)
出处
《天津农业科学》
CAS
2018年第11期1-3,共3页
Tianjin Agricultural Sciences
关键词
干旱胁迫
西瓜
酶活
叶绿素荧光
根系活力
drought stress
watermelon
enzyme activity
chlorophyll fluorescence
root activity