摘要
目的探讨程序性死亡配体1(PD-L1)在三阴性乳腺癌(TNBC)中的表达情况及其与PTEN基因的关系。方法通过癌症基因组图谱(TCGA)数据库查询PD-L1 mRNA在浸润性乳腺癌数据集(包括115例TNBC和702例非TNBC)中的表达情况。收集2012年1月至2016年12月解放军第180医院收治的182例浸润性乳腺癌术后石蜡包埋组织标本,包括62例TNBC和120例非TNBC,并用免疫组织化学方法检测PD-L1和PTEN的表达情况。用t检验比较TCGA数据库中2组的PD-L1 mRNA表达量,率的比较用χ~2检验,Mann-Whitney U秩和检验比较石蜡标本中2组PD-L1表达强度,并用χ2检验和Spearman秩相关检验分析PD-L1与PTEN表达的相关性。结果 TCGA数据库分析显示,浸润性乳腺癌中3. 8%(31/817)有PD-L1 mRNA上调,其中TNBC组的表达上调率为8. 7%(10/115),高于非TNBC组的3. 0%(21/702)(χ~2=7. 314,P=0. 007),TNBC组的PD-L1 mRNA表达量为8. 05±0. 91,高于非TNBC组的7. 38±0. 73 (t=7. 510,P<0. 001)。石蜡标本的免疫组织化学结果显示,TNBC组的PDL1阳性表达率为14. 5%(9/62),高于非TNBC组的5. 0%(6/120)(χ~2=4. 895,P=0. 027),而且PD-L1阳性表达强度也高于非TNBC组(Z=-2. 291,P=0. 022)。TNBC石蜡标本中PD-L1与PTEN蛋白表达呈负相关(χ~2=6. 913,P=0. 009; r=-0. 382,P=0. 002)。结论 TNBC中PD-L1表达高于非TNBC,并与PTEN负相关,其可能成为TNBC患者的免疫治疗靶点。
ObjectiveTo investigate the expression of programmed death ligand 1（PD-L1） in triple negative breast cancer （TNBC） and its relationship with PTEN expression.Methods In the Cancer Genome Atlas （TCGA） database, the expression of PD-L1 mRNA in the invasive breast cancer dataset was analyzed, including 115 TNBC cases and 702 non-TNBC cases. A total of 182 paraffin-embedded tissue specimens from invasive breast cancer patients admitted to the No.180 Hospital of PLA from January 2012 to December 2016 were collected and the expression of PD-L1 and PTEN was immunohistochemically detected. PD-L1 mRNA expression was compared between two groups in TCGA database using t test.χ 2 test was used to compare the rates.The intensity of PD-L1 positive expression in paraffin-embedded specimens were compared between two groups using Mann-Whitney U test. χ 2 test and Spearman rank test were used to analyze the correlation between PD-L1 and PTEN expression.Results In TCGA database, PD-L1 mRNA level was up-regulated in 3.8% （31/817） of invasive breast cancer. The up-regulation rate of PD-L1 mRNA in TNBC （8.7%,10/115） was significantly higher than that in non-TNBC （3.0%,21/702） （χ 2=7.314, P =0.007）. PD-L1 mRNA expression in TNBC was significantly higher than that in non-TNBC（8.05±0.91 vs 7.38±0.73, t =7.510, P 〈0.001）. The immunohistological results of 182 paraffin-embedded tissue specimens showed that the positive expression rate of PD-L1 in TNBC （14.5%,9/62） was significantly higher than that in non-TNBC（5.0%, 6/120 ）（χ 2=4.895, P =0.027）, and the expression intensity of PD-L1 in TNBC was significantly higher than that in non-TNBC（ Z =-2.291, P =0.022）. The PD-L1 protein expression was negatively correlated with PTEN protein expression in paraffin-embedded TNBC specimens （χ 2=6.913, P = 0.009, r =-0.382, P =0.002）.Conclusion PD-L1, which shows higher expression in TNBC patients than in non-TNBC patients and a negative correlation with PTEN, may be a potential target for TNBC
作者
魏开鹏
陈海莺
陈燕红
邱建龙
Wei Kaipeng;Chen Haiying;Chen Yanhong;Qiu Jianlong(Department of Pathology,2Department of Surgery,No.180 Hospital of PLA,Quanzhou 362000,China)
出处
《中华乳腺病杂志（电子版）》
CAS
CSCD
2018年第5期294-298,共5页
Chinese Journal of Breast Disease(Electronic Edition)
