摘要
目的:对比四氯化碳(carbon tetrachloride,CCl4)诱导的ICR小鼠和C57小鼠的肝纤维化模型差异。方法:雄性ICR小鼠和C57BL小鼠各30只,ICR小鼠随机分为ICR正常组、ICR阴性对照组(橄榄油)、ICR模型组; C57BL小鼠随机分为C57正常组、C57阴性对照组和C57模型组,每组10只。采用20%的CCl4橄榄油混悬液腹腔注射建立肝纤维化模型。检测血清生化、常规病理HE染色和天狼星红染色观察各组肝功和病理变化,Western Blot和荧光定量PCR检测肝细胞外纤维化表达程度。结果:肝功能方面,两种小鼠模型组谷丙转氨酶和谷草转氨酶均显著高于其对照组和正常组,差异有统计学意义(P <0. 05),但对照组与正常组之间差异无统计学意义(P>0. 05)。病理上,两种小鼠模型组肝小叶被破坏、肝索紊乱,其中ICR小鼠模型组可见肝细胞弥漫性脂肪变性,间质纤维化形成明显。模型组肝间质成分表达上,ICR小鼠肝纤维化模型α-SMA、Collagen I、Collagen III和Fibronectin蛋白均显著高于C57小鼠,差异有统计学意义(P <0. 05); ICR小鼠肝组织中α-SMA、Collagen I和Collagen III mRNA的表达也明显高于C57小鼠,差异有统计学意义(P <0. 05)。结论:CCl4在两种小鼠均可建立典型的肝纤维化模型,但ICR小鼠比C57小鼠肝纤维化更明显。
Objective To compare the differences of liver fibrosis model between ICR and C57 mice induced by carbon tetra- chloride （carbon tetrachloride, CCl4）. Method The group of ICR mice and C57 mice were both Males and each group were both randomly divided into normal group, vehicle group （olive oil） and model group, and each group with 10 mice. 20% of CCl4 olive oil mixed suspension were injected to abdominal cavity to establish hepatic fibrosis model. The liver function and pathological changes were reacted through serum bioehenfieal, pathological HE and Sirius red staining. The quantitative expression of hepatic extraeellular fibrosis was reacted through Western Blot and fluorescence quantitative PCR. Results On the liver function, ALT and AST in two groups of mouse models were both significantly higher than those in the normal group and the vehicle group （ P 〈 0. 05 ） , but there was no significant difference between the two model groups （ P 〉 0. 05 ）. On the pathology-,liver lobules and liver cords were destroyed and disordered in both model groups,in which diffuse steatosis and interstitial fibrosis of liver cells were ob- vious of ICR nfiee. On the expression of liver stromal component in both model groups, the proteins of ot - SMA, Collagen I , Col- lagen III and Fibroneetin in ICR mice were significantly higher than those in C57 nfiee（ P 〈 0. 05 ） ;the mRNA expression of α - SMA, Collagen [ and Collagen III in ICR nfiee was also significantly higher than that of C57 nfiee（ P 〈 0. 05 ）. Conclusion CCl4 can be used to establish the typical model of liver fibrosis in both kinds of mice, while ICR mice were more obvious than C57 mice in liver fibrosis.
作者
钟霞
王丽
王洪连
沈宏春
徐川岚
刘育杭
ZHONG Xia;FANG Li;FANG Hong-lian(Research Center of Integrated Chinese and Western Medicine,Affiliated Tra-ditional Medicine Hospital of Southwest Medical University,Luzhou 646000,China;Southwest Medical University,Luzhou 646000,China)
出处
《吉林医学》
CAS
2018年第10期1806-1809,共4页
Jilin Medical Journal
基金
西南医科大学附属中医医院-西南医科大学联合课题[项目编号:(2016)4号]
四川省教育厅重点项目[项目编号:(2014)794号]
西南医科大学院级课题[项目编号:2014QN-011]
