摘要
目的建立中药复方戒毒一号的高效液相指纹图谱,为戒毒一号的质量控制提供依据。方法采用高效液相色谱(HPLC)法,Thermo公司Ultimate TM XB-C18色谱柱,延胡索甲素、延胡索乙素、小檗碱、甘草酸以乙腈-水-磷酸缓冲液为流动相,体积流量为1. 0 mL/min,柱温30℃,检测波长为280 nm;戒毒一号中的人参皂苷Re、人参皂苷Rb1以乙腈-纯水为流动相,二元梯度洗脱,体积流量为1. 0 m L/min,柱温30℃,检测波长203 nm。结果 10批戒毒一号水煎剂中共有峰的相对保留时间RSD小于1%。在选定的色谱条件下确定了19个峰为戒毒一号的特征指纹峰,10批样品平均相似度为0. 988。结论该法重现性好,可为戒毒一号的质量控制提供依据。
Objective The aim of this study is to develop the fingeqorints of the Jieduyihao （JDYH） with high performance liquid chromatographic （HPLC） analysis and to provide the basis for the quality control of JDYH. Methods The HPLC fingeprints were conducted with the Ultimate TM XB-C18 （250 mm × 4.6 mm） colunm in gradient elution. The analyses of corydaline, tetrahydropalmatine, berberine and glycyrrhizic acid were conducted with the mobile phase consisted of acetonitrile-water-phosphoric acid, at column temperature 30 ℃, flow rate of 1.0 mL/min and detective wavelengths of 280 nm. The analyses of ginsenoside Re and ginsenoside Rbl were conducted with the mobile phase consisted of acetonitrile-water in gradient elution, at column temperature 30 ℃, flow rate of 1.0 mL/rain and detective wavelengths of 203 nm. Results The fingeqorints of ten batches of JDYH decoction were established, which contained 19 common peaks, the similarities among different batches were 0.998, with RSD 〈 1%. Conclusion The analyses of HPLC fingerprints have high sensitivity and good reproducibility, which can provide a basis for scientific evaluation and quality control of JDYH.
作者
李灿
唐利军
茹琴
周梅
李超英
LI Can;TANG Lijun;RU Qin;ZHOU Mei;LI Chaoying(Wuhan Institutes of Biomedical Sciences,Jianghan University,Wuhan 430056,Hubei,China;Hubei Provincial Center for Disease Control And Prevention,Wuhan 430073,Hubei,China)
出处
《江汉大学学报（自然科学版）》
2018年第5期471-476,共6页
Journal of Jianghan University：Natural Science Edition
基金
武汉市首批黄鹤英才计划预防毒品复吸新药的研发与推广项目
抗冰毒复吸中药组方的药效及毒理学临床前研究(1018-06010009)
江汉大学研究生科研创新基金项目(301004210001)
关键词
戒毒一号
指纹图谱
高相液相色谱
Jieduyihao （JDYH）
fingerprint
high performance liquid chromatography （HPLC）
