摘要
目的探讨酸性成纤维细胞生长因子(aFGF)对脂多糖诱导的大鼠急性肺损伤(ALI)的作用及其影响机制。方法将60只雄性SD大鼠按照随机数字表法分为3组:阴性对照组、脂多糖损伤组、aFGF干预组,每组20只。采用经气管插管给予脂多糖5 mg·kg-1制备大鼠ALI模型,aFGF干预组则于给予脂多糖24 h后,经气道给予aFGF 1 000μ·g-1。3组均于48 h后苏木精-伊红染色观察肺组织病理学改变,计算肺损伤评分,测定肺水清除率,称量肺湿/干重比,免疫组织化学染色法观察肺泡表面活性蛋白C(SP-C)的表达,酶联免疫吸附试验法测定肺泡灌洗液中肿瘤坏死因子α(TNF-α)的水平。蛋白质印迹法检测肺组织磷酸化p38丝裂原活化蛋白激酶(p-p38MAPK)蛋白表达水平。数据采用单因素方差分析(进一步两两比较采用LSD法)或非参数检验。结果 (1) aFGF干预组肺损伤评分[(6. 33±0. 42)分]与脂多糖损伤组肺损伤评分[(11. 00±0. 37)分]较阴性对照组[(1. 01±0. 26)分]均升高,但aFGF干预组肺损伤评分明显低于脂多糖损伤组,差异有统计学意义(P <0. 05)。(2) aFGF干预组肺水清除率[(27. 41±1. 05)%]与脂多糖损伤组肺水清除率[(15. 59±0. 64)%]较阴性对照组[(30. 63±0. 91)%]均有所下降,但aFGF干预组明显高于脂多糖损伤组,差异均有统计学意义(P <0. 05)。(3)脂多糖损伤组肺水聚集(肺湿/干重比为6. 32±0. 32)较阴性对照组(4. 17±0. 05)明显升高,aFGF干预组(5. 18±0. 10)也高于阴性对照组,但与脂多糖损伤组相比,肺湿/干重比有所改善且差异有统计学意义(P <0. 05)。(4) aFGF干预组TNF-α水平[(762. 50±23. 93) pg/m L]与脂多糖损伤组TNF-α水平[(1460. 01±17. 96) pg/m L]较阴性对照组[(49. 51±10. 75) pg/m L]均明显升高,但aFGF干预组较脂多糖损伤组有明显下降,差异均有统计学意义(P <0. 05)。(5) aFGF干预组p-p38 MAPK/β-actin比值(0. 38±0. 01)明显高于阴性对照组(0. 18±0. 01),差异有统计学�
Objectives To investigate the effect of acid fibroblast growth factor （aFGF） on lipopolysaccharides-induced acute lung injury in rat. Methods Sixty male SD rats were divided into three groups according to the random number table： negative control group, lipopolysaccharide injury group and aFGF intervention group, with 20 rats in each group. Acute lung injury model of rats was prepared using lipopolysaccharide 5 mg · kg -1 via endotracheal intubation, and aFGF intervention group received aFGF 1 000 μ·g- 1 via airway after 24 hours of lipopolysaccharide administration. Three groups are in 48 h after hematoxylin eosin staining to observe the lung tissue pathology change, lung injury score calculation, the determination of lung water clearance, weighing lung wet/dry weight ratio （ W/D ） , immunohistochenfical staining to observe the alveolar surface activated protein C （SP-C） expression of enzyme-linked inmmnosorbent assay method determination of tumor necrosis factor alpha （TNF-α） in alveolar lavage levels. The protein expression level of p38 mitogen-activated protein kinase （p-p38MAPK） in lung tissue was detected by protein imprinting. The data were analyzed by single factor anova （ LSD method for further pairwise comparison） or non-parametric test. Results （1） Among the lung injury scores, both the aFGF intervention group [ （6. 33 ± 0. 42） points ] and the lipopolysaccharide injury group [ （ 11. 00 ± 0.37） points] were increased compared with the negative control group [（ 1.01 ±0.26） points], but the lung injury score of the aFGF intervention group was significantly lower than the lipopolysaccharide injury group, the differences were statistically significant （P 〈 0.05）. （2） In terms of lung water clearance rate, the level of AFC in aFGF group [ （27.41 ± 1.05） % ] and the lipopolysaccharide injury group [ （ 15.59 ± 0.64） % ] were decreased compared with the negative control group [ （30.63 ± 0.91 ）% ], but the aFGF group was significantly hig
作者
李世元
岳红云
张湘华
罗园
Li Shiyuan;Yue Hongyun;Zhang Xianghua;Luo Yuan(Department of Burns;Department of Respiratory,Affiliated People＇s Hospital of Hebei Medical University,Shijiazhuang 050011,China)
出处
《中华损伤与修复杂志（电子版）》
CAS
2018年第5期342-347,共6页
Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)
基金
河北省医学科学研究重点课题(20181000)
关键词
成纤维细胞生长因子1
急性肺损伤
脂多糖类
肺水清除率
Fibroblast growth factor 1
Acute lung injury
Lipopolysaccharides
Alveolar fluid clearance
