摘要
目的优化葛根的超声提取工艺。方法采用正交试验法,以水为溶剂,以葛根素、大豆苷元为考察指标,对葛根超声提取工艺中的溶剂用量、单次提取时间及提取次数进行优化。结果葛根素进样量线性范围为0. 024~0. 160μg;稳定性的RSD为1. 00%(n=6);精密度的RSD为0. 76%(n=6);重复性的RSD为0. 80%(n=6);回收率为99. 87%,RSD为0. 70%(n=6)。大豆苷元进样量线性范围为0. 03~0. 20μg;稳定性的RSD为1. 10%(n=6);精密度的RSD为0. 76%(n=6);重复性的RSD为0. 90%(n=6);回收率为100. 32%,RSD为1. 80%(n=6)。最佳超声提取工艺为加8倍量水,提取3次,每次30 min。结论该方法简便,准确,专属性强,工艺重复性好,适于规模化生产。
Objective To optimize the ultrasonic extraction process of Puerariae lobatae Radix. Methods With the water as solvent,puerarin and daidzein as indexes,the amount of solvent,single extraction time and extraction times in the ultrasonic extraction process of Puerariae lobatae Radix were optimized by orthogonal test. Results The puerarin showed a good linear relationship in the range of0. 024-0. 160 μg,the RSD of stability was 1. 00%（ n = 6）,the RSD of precision was 0. 76%（ n = 6）,the RSD of repeatability was 0. 80%（ n = 6）,the recovery rate was 99. 87%,RSD was 0. 70%（ n = 6）. The daidzein showed a good linear relationship in the range of 0. 03-0. 20 μg,the RSD of stability was 1. 10%（ n = 6）,the RSD of precision was 0. 76%（ n = 6）,the RSD of repeatability was0. 90%（ n = 6）,the recovery rate was 100. 32%,RSD was 1. 80%（ n = 6）. The best ultrasonic extraction process was as follows： eight times the amount of water,extracting 3 times,each time 30 min. Conclusion This method is simple,accurate with strong specificity and good repeatability,which is suitable for large-scale production.
作者
崔颖
刘馨湄
才凤
Cui Ying;Liu Xinmei;Cai Feng(Liaoning Academy of Traditional Chinese Medicine,Shenyang,Liaoning,China 110034;Liaoning Institute for Food Control,Shenyang,Liaoning,China 110015)
出处
《中国药业》
CAS
2018年第20期7-9,共3页
China Pharmaceuticals
关键词
葛根
含量测定
超声提取工艺
正交设计
葛根素
大豆苷元
Puerariae lobatae Radix
content determination
ultrasonic extraction process
orthogonal test
puerarin
daidzein
