摘要
【目的】研究膜联蛋白A1(Annexin A1,ANXA1)对Ⅰ型干扰素(I-IFN)表达及口蹄疫病毒(FMDV)复制的影响。【方法】开展过表达及Knockdown实验,检测ANXA1对FMDV复制的影响。利用双荧光素酶报告系统检测ANXA1对ISRE和IFN-β启动子元件活化的影响。双荧光素酶报告系统鉴定ANXA1调控Ⅰ-IFN通路活化靶分子。Western blotting检测ANXA1对干扰素调解因子3(IRF3)磷酸化的影响。Real-time PCR检测ANXA1对干扰素刺激基因(ISGs)的影响。【结果】过表达ANXA1显著抑制FMDV的复制;下调ANXA1表达促进FMDV复制(P<0.01或P<0.05);ANXA1促Ⅰ型干扰素通路活化,呈现剂量依赖性(P<0.01)。ANXA1显著增强IRF3的磷酸化,促进ISGs的表达(P<0.01或P<0.05)。【结论】ANXA1促进Ⅰ-IFN表达,抑制FMDV的复制。
[Objective] We studied the regulatory effect of Annexin-1（ANXA1） on type Ⅰ interferon（Ⅰ-IFN） production and foot-and-mouth disease virus（FMDV） replication. [Methods] We used overexpression and knockdown assays to determine the role of ANXA1 in FMDV-infected cells. Then we evaluated the influence of ANXA1 on ISRE and IFN-β promoter activation by dual luciferase reporter assays. To confirm the regulatory role of ANXA1 on type Ⅰ IFN pathway, IFN regulatory factor 3（IRF3） phosphorylation was detected by Western blotting. Expression of interferon-stimulated genes（ISGs） was measured by RT-PCR to investigate the effect of ANXA1 on ISGs expression. [Results] Overexpression of ANXA1 significantly suppressed FMDV replication and knockdown of ANXA1 expression enhanced virus replication, showing an antiviral role of ANXA1 in FMDV-infected cells（P〈0.01 or P〈0.05）. ANXA1 promoted the activation of type Ⅰ IFN pathway in a dose-dependent manner. Overexpression of ANXA1 enhanced IRF3 phosphorylation and ISGs expression（P〈0.01 or P〈0.05）. [Conclusion] ANXA1 enhances type Ⅰ IFN production and suppresses FMDV replication.
作者
罗志宽
马旭升
杨孝朴
郑海学
Zhikuan Luo1,2, Xusheng Ma2, Xiaopu Yang1, Haixue Zheng2.(1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China ;2. State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu Province, China)
出处
《微生物学报》
CAS
CSCD
北大核心
2018年第10期1798-1807,共10页
Acta Microbiologica Sinica
基金
国家自然科学基金(31602037)
农业部家"948项目"(2015-Z6)~~
