摘要
目的:探讨DNMT3L(DNA methyltransferase 3-like)蛋白在不同种类卵巢恶性生殖细胞肿瘤中的表达及其在生殖细胞肿瘤发生发展过程中的作用。方法:收集卵巢恶性生殖细胞肿瘤59例,包括原始生殖细胞肿瘤52例及未成熟性畸胎瘤7例,原始生殖细胞肿瘤包括胚胎性癌10例,卵黄囊瘤22例,无性细胞瘤13例,混合性生殖细胞肿瘤7例;同时收集非生殖细胞肿瘤17例,包括浆液性癌5例,颗粒细胞瘤5例,卵泡膜细胞瘤7例,采用SP免疫组织化学方法检测肿瘤组织中的DNMT3L蛋白的表达情况,并结合临床病理特征及组织学类型进行统计学分析。结果:DNMT3L主要在原始生殖细胞肿瘤表达,总体阳性率为73.1%(38/52),其中混合性生殖细胞肿瘤阳性表达率为85.7%(6/7),无性细胞瘤阳性表达率为84.6%(11/13),卵黄囊瘤中阳性表达率为68.2%(15/22),胚胎性癌阳性表达率为60%(6/10)。DNMT3L在7例未成熟畸胎瘤及17例非生殖细胞肿瘤中均未见阳性表达。临床病理特征分析显示DNMT3L阳性表达率与原始生殖细胞肿瘤的FIGO分期有关,与年龄及肿瘤大小等无关。结论:DNMT3L在原始生殖细胞肿瘤阳性表达率高,对其诊断具有较好的敏感性和特异性,DNA甲基化的改变可能在卵巢原始生殖细胞肿瘤的发生过程中起到重要作用。
Objective： To investigate the expression patterns of DNA methyltransferase 3-like（DNMT3L） protein in malignant ovarian germ cell tumors and the underlying roles in the pathogenesis of germ cell tumors. Methods： A total of 59 cases of ovarian malignant germ cell tumors were collected which include 52 cases primitive germ cell tumors and 7 cases immature teratoma. Primitive germ cell tumors included 10 cases embryonal carcinoma, 22 cases yolk sac tumors, 13 cases dysgerminoma, and 7 cases mixed germ cell tumors; meanwhile 17 cases non-germ cell tumors were collected including 5 cases serous adenocarcinoma, 5 cases granulose cell tumors and 7 cases. SP immunohistochemistry was performed to detect the DNMT3L expression in tumor tissues. The relationship with clinical-pathologic features and histological categories were analyzed. Results： DNMT3L was mainly expressed in primitive germ cell tumors, with total 73.1%（38/52） positive expression, 85.7%（6/7） positive expression for mixed germ cell carcinoma, 84.6%（11/13） for dysgerminoma, 68.2%（15/22） for yolk sac tumors and 60%（6/10） for embryonal carcinoma. The expression of DNMT3L was negative in 7 cases immature teratoma and 17 cases non-germ cell carcinoma. DNMT3L-positive proportion correlated to FIGO staging and had no correlations to age and tumor size. Conclusion： DNMT3L has a high positive rate in the primitive germ cell tumor and has a good sensitivity and specificity for its diagnosis. The change of DNA methylation may play an important role in the process of ovarian primitive germ cell tumor.
作者
丁晓燕
张采欣
周磊
孔庆暖
DING Xiaoyan;ZHANG Caixin;ZHOU Lei;KONG Qjngnuan(Department of Pathology,Affiliated Hospital of Qingdao University,Qingdao Shandong 266011;Department of Pathology,Qingdao Municipal Hospital,Qingdao Shandong 266071,China)
出处
《临床与病理杂志》
2018年第8期1608-1613,共6页
Journal of Clinical and Pathological Research
基金
青岛市医疗卫生重点学科建设项目(YQ2017Y17)~~
