摘要
目的探讨针刺对短暂性脑缺血(TIA)发作大鼠神经元凋亡、腺苷水平的影响。方法 Wistar雄性大鼠32只,随机分为4组,分别为:正常对照组(8只)、假手术组(8只)、模型组(8只)、针刺组(8只)。全脑缺血TIA大鼠模型利用4血管阻断法建立。针刺组造模后给予电针治疗,其余三组不给予任何治疗,术后1周后取材。原位末端标记法(TUNEL)染色检测大脑皮质、海马CA1区神经元凋亡,分光光度计测定大脑皮质、海马区腺苷含量。结果神经元凋亡:模型组CA1区、大脑皮质为(36.44±8.96)、(36.94±8.77)个/mm3,高于针刺组的(18.52±6.32)、(20.11±6.70)个/mm^2,差异有统计学意义(P<0.05)。正常对照组(11.54±2.68)、(10.23±3.19)个/mm^2、假手术组(13.98±5.47)、(11.63±4.55)个/mm^2均低于模型组,差异有统计学意义(P<0.05)。腺苷含量:模型组CA1区、大脑皮质为(0.139±0.016)、(0.140±0.017)μmol/L mg,低于针刺组(0.176±0.004)、(0.178±0.006)μmol/L mg,差异有统计学意义(P<0.05)。正常对照组[(0.038±0.006)、(0.039±0.009)μmol/L mg]、假手术组[(0.037±0.006)、(0.038±0.005)μmol/L mg]均低于模型组,差异有统计学意义(P<0.05)。结论针刺可以抑制TIA发作模型大鼠神经元凋亡,增加内源性腺苷含量。
Objective To study the effect of acupuncture on apoptosis and adenosine level of neurons in rats with tran- sient ischemic attack （TIA）. Methods 32 male Wistar rats are randomly divided into 4 groups, norMal control group （8 rats）, sham-operation group （8 rats）, model group （8 rats）, acupuncture group （8 rats）. Global cerebral ischemia TIA rat model was established by four-vessel occlusion. The acupuncture group was adminstered with electro acupuncture ther- apy and other 3 groups without any other treatment, to sample 1 week after surgery. By the TUNEL dyeing test （In situ terminal labeling） to detect the Neuron apoptosis in cerebral cortex and hippocampus CA1 area, and determine Adeno- sine content in cerebral cortex and hippocampus area by spectrophotometer, Results The neuronal apoptosis in the CA1 area and cerebral cortex of the model group were （36.44±8.96）, （36.94±8.77） single/ram2 higher than that of the acupuncture group （18.52±6.32）, （20.11±6.70）single/ram2 with significant difference （P〈0.05）. As for normal control group and the sham operation group, the values were （13.98±5.47）, （11.63±4.55）single/ram2 both lower than that of model group, with statistical difference （P〈0.05）. Adenosine content： the CA1 area and cerebral cortex of the model group were （0.139±0.016） and （0.140±0.017） ]_Lmol/L nag, lower than that of the acupuncture group （0.176±0.004） （0.178± 0.006） ]_Lmol/L rag, with statistical difference （P〈0.05）. As for normal control group （0.038±0.006）, （0.039±0.009） ]_Lmol/L mg and the sham operation group （0.037±0.006）, （0.038±0.005）]μmol/L mg were lower than the model group, and the dif-ference was statistically significant （P〈0.05）. Conclu- sion Acupuncture can inhibit the apoptosis of neurons in TLA rats and increase the content of endogenous adeno- sine in them.
作者
张军
刘敏
唐伟
王威
ZHANG Jun;LIU Min;TANG Wei;WANG Wei(Department of Neurology,Affiliated Xinhua Hospital of Dalian University,Liaoning Province,Dalian 116021,China;Department of Rehabilitation,Affiliated Zhongshan Hospital of Dalian University,Liaoning Province,Dalian 116001,China)
出处
《中国当代医药》
2018年第27期32-34,共3页
China Modern Medicine
基金
辽宁省大连市中医药相关科学研究计划项目(大卫办发[2016]133号)
关键词
短暂性脑缺血发作
针灸疗法
细胞凋亡
腺苷
Transient isehemic attack
Acupuncture therapy
Apoptosis
Adenosine
