摘要
目的探究大黄酚(chrysophanol,CP)对幼龄大鼠非酒精性脂肪肝(non-alcoholic fatty liver disease,NAFLD)的调节作用。方法将40只大鼠随机分为对照(control,Ctrl)组、CP组,高脂(high-fat,HF)组和HF+CP组。HF组和HF+CP组用高脂饲料喂养,其余2组以正常饲料喂养,CP组和HF+CP组同时腹腔注射CP(30 mg/kg)。4周后收集各组大鼠血清及肝脏组织,HE染色检测肝脏病理情况、TUNEL法检测细胞凋亡、Western blot检测脂质合成和分解代谢基因的表达、ELISA检测血清炎症因子水平。结果与Ctrl组比较,HF组大鼠肝脏组织出现明显的肝脂肪变性及炎性细胞浸润,凋亡小体明显增多;与HF组比较,HF+CP组大鼠肝组织脂肪变性及炎性细胞浸润显著减轻,凋亡小体数明显减少;同时,与Ctrl组比较,HF组大鼠脂质合成代谢基因ACC和SPEBP-1c的表达水平明显升高,分解代谢基因PPARα和CPT-1的表达水平明显降低,CP能显著减弱HP对ACC、SPEBP-1c、PPARα和CPT-1表达的影响。此外,HF能显著升高大鼠血清IL-1β和IL-6水平,CP能显著降低模型大鼠血清IL-1β和IL-6水平。结论 CP能通过调控脂质的合成代谢及炎症反应减轻幼龄大鼠NAFLD。
To investigate the effects of chrysophanol （CP） on high fat （HF） diet-induced non-alcoholic fatty liver disease （NAFLD） in neonatal rats, total of 40 rats were recruited and randomly divided into control （Ctrl）, CP, HF and HF＋CP groups. The rats in HF and HF＋CP groups were fed with high fat diet, while the rats in the other two groups were fed with normal diet. The rats in CP and HF＋CP group were intraperitoneally injected with CP （30 mg/ kg） at the same time. Four weeks after, the serum and liver issue were collected and HE staining was performed for pathological injury observation. Cell apoptosis was determined by TUNEL assasy, and the expressions of lipid synthesis and lipidolysis-related genes were measured by Western blotting. Furthermore, the levels of inflammatory cytokines in serum were determined by ELISA. Data showed that compared with Ctrl group, the liver of rats in HF group characterized with steatosis and inflammatory cell infiltrates, and the numbers of apoptotic body increased. Compared with HF group, the steatosis and inflammatory eel1 infiltration were alleviated significantly in rats of HF＋ CP group, and the number of apoptotic body was decreased significantly. Meanwhile, the expression levels of ACC and SPEBP-1c were increased, while PPARα and CPT-1 levels were decreased in HF group as compared with Ctd group, while CP could reversed these changes in HE group markedly. In addition, the serum levels of IL- 1β and IL-6 were up-regulated by HF, while CP down-regulated the concentrations of IL-1β and IL-6 of model rats notably. In conclusion, CP attenuates NAFLD in neonatal rats via regulating lipid synthesis, lipidolysis and inflammation.
作者
胡云芝
李军民
鲁之中
李卫
姜富国
桂静
HU Yunzhi;LI Junmin;LU Zhizhong;LI Wei;JIANG Fuguo;GUI Jing(Department of Pediatrics,Jiaozuo People＇s Hospital in Henan Province,Jiaozuo 454002,China;Department of Clinical Laboratory,Jiaozuo People＇s Hospital in Henan Province,Jiaozuo 454002,China)
出处
《免疫学杂志》
CAS
CSCD
北大核心
2018年第10期869-874,共6页
Immunological Journal
基金
河南省2015年科技发展计划(152300410164)
关键词
非酒精性脂肪肝
脂质代谢
炎症
Non-alcoholic fatty liver disease
Lipid metabolism
Inflammation
