摘要
目的:研究茵杞调脂饮改善非酒精性脂肪肝病(NAFLD)大鼠脂质代谢的作用及机制。方法:48只雄性SD大鼠随机分为正常组,模型组,茵杞调脂饮低、中、高剂量组及辛伐他汀对照组,各8只。正常组喂养普通饲料,其余组喂养高脂饲料,12周后模型组灌胃0.9%氯化钠溶液,各药物组灌胃相应剂量药物,8周后检测血清ALT、AST、TC、TG及肝组织TC、TG、LP、TNF-α含量,HE、油红O染色检测肝组织脂肪变性程度,RT-PCR检测肝组织LXRα、FAS mRNA表达。结果:与正常组比较,模型组大鼠血清ALT、AST、TC、TG及肝组织TC、TG、LP、TNF-α,LXRα、FAS mRNA显著升高(P<0.01),肝组织出现显著的细胞脂肪变性及空泡样变;与模型组比较,各药物组大鼠血清ALT、AST、TC、TG及肝组织TC、TG、LP、TNF-α,LXRα、FAS mRNA显著降低(P<0.05,P<0.01),肝细胞脂肪变性及空泡样变得到明显改善;与辛伐他汀组比较,茵杞调脂饮高剂量组血清ALT、AST,肝组织LP、TNF-α及LXRα、FAS mRNA显著降低(P<0.05)。结论:茵杞调脂饮可以调节NAFLD大鼠的脂代谢,减少脂质沉积,减轻氧化应激,其机制可能与抑制LXRα、FAS的表达有关。
Objective:To investigate the effects of Yinqi Tiaozhi Decoction on improving lipid metabolism in rats with non-alcoholic fatty liver disease(NAFLD).Methods:Forty-eight healthy male SD rats were randomly divided into normal group,model group,low-dose,middle-dose and high-dose Yinqi Tiaozhi Decoction group and Simvastatin positive drug control group,8 rats each group.The normal group received standard diet and the rest groups were fed with high-fat diet.After 12 weeks,the model group was given normal saline,and drug groups was given the corresponding dose of drugs.After 8 weeks,all of the rats were sacrificed.The observing items including ALT,AST,TC and TG levels in serum;TC,TG,TNF-α,LP content in liver tissue;HE staining and oil red O staining were used to detecting the degree of hepatic steatosis;RT-PCR testing LXRαand FAS mRNA expression in liver.Results:Compared with normal group,the levels of ALT,AST,TC,TG in serum,TC,TG,LP,TNF-αand LXRα,FAS mRNA in liver in the model group were remarkably elevated(P<0.01);liver tissue showed significant hepatocyte steatosis and vacuolar degeneration.Compared with the model group,drug groups of levels of ALT,AST,TC,TG in serum,TC,TG,LP,TNF-αand LXRα,FAS mRNA in liver were decreased(P<0.05,P<0.01);hepatocyte steatosis and vacuolar degeneration improved significantly.Compared with the simvastatin group,the high-dose group of Yinqi Tiaozhi Decoction showed a significant efficacy in serum ALT,AST,and liver tissue LP,TNF-α,LXRα,FAS mRNA decreased significantly(P<0.05).Conclusion:Yinqi Tiaozhi Decoction can regulate lipid metabolism,reduce lipid deposition and oxidative stress in rats with nonalcoholic fatty liver disease.The mechanism may be related to the inhibition of LXRαand FAS expression.
作者
王俐钧
孙建光
WANG Li-jun;SUN Jian-guang(Shandong University of Traditional Chinese Medicine,Jinan250014,China;Affiliated Hospital ofShandong University of Traditional Chinese Medicine,Jinan250014,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2019年第10期4566-4570,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
山东省高等学校科技计划项目(No.J09LF14)~~
