摘要
本文以多花黄精顶芽为外植体开发了一种多花黄精组培快繁的新方法。研究发现,外植体经75%的酒精结合10%的NaClO消毒后,污染率为4±0. 08%。文中研究了1/2 MS培养基添加不同浓度(0.1～4 mg·L^(-1))的细胞分裂素(6-BA、2,4-D及TDZ)及不同浓度生长素(NAA及IBA)对外植体愈伤组织诱导、不定芽分化及不定芽生根的影响。结果表明:外植体愈伤组织诱导及不定芽分化的最佳培养基组合为1/2 MS+6-BA(3 mg·L^(-1))+TDZ(0. 5 mg·L^(-1))+NAA(0. 2 mg·L^(-1)),不定芽最佳生根培养基组合为1/2 MS+NAA(0. 4 mg·L^(-1))。应用此繁殖方法,将一个顶芽组织培养繁殖6个月后可获得约80株组培苗。组培苗移植到温室后成活率为(96±1. 3)%。
An ideal micropropagation method for Polygonatum cyrtonema Hua has been developed using terminal buds as explants. The explants were sterilized with 75% ethyl alcohol plus 10% sodium hypochlorite instead of mercuric chloride, and the resulting contamination rate was 4±0. 08%. All the explants were cultured on 1/2 Murashige and Skoog medium supplemented with different concentrations（0. 1~4 mg·L^-1）and combinations of cytokinins（6-benzyladenine, 2,4-D-ethylhexyl and thidiazuron）along with auxins（1-naphthaleneacetic acid and indole-3-butyric acid）for callus, adventitious bud and root induction. The optimum hormone combination for callus and adventitious bud induction was 3. 0 mg·L^-1 6-benzyladenine,0. 5 mg·L^-1 thidiazuron and 0. 2 mg·L^-1 1-naphthaleneacetic acid. For root induction,1/2 Murashige and Skoog medium supplemented with 0.4 mg·L^-1 1-naphthaleneacetic acid was optimum. Almost 80 plantlets were regenerated from one terminal bud after it was micropropagated in an in vitro culture for 6 monthes. Regenerated plantlets were successfully transferred to a greenhouse and had a survival rate of 96±1. 3%.
作者
沈宝明
杨硕知
谭著明
申爱荣
谭云
SHEN Baoming;YANG Shuozhi;TAN Zhuming;SHEN Airong;TAN Yun(Hunan Academy of Forestry,Changsha 410004,China;Hunan Engineering Technology for Culturing and Utilizing of Understorey Bioresources,Changsha 410004,China)
出处
《湖南林业科技》
2018年第5期27-31,共5页
Hunan Forestry Science & Technology
基金
湖南省林业项目"黄精组织培养规模化繁殖技术研究"
国家重点研发计划(2017YFD0600302)
关键词
植株再生
无汞消毒剂
多花黄精
顶芽
plantlet regeneration
HgCl2-free disinfector
Polygonatum cyrtonema Hua
terminal buds
