摘要
为建立最佳的马铃薯块茎再生体系,本研究分别以‘夏坡蒂’、‘费乌瑞它’以及‘克新1号’的试管薯和大田薯为试验材料,采用控制变量法对马铃薯块茎再生体系进行筛选,以获得其培养基最佳激素浓度配比,并对不同来源马铃薯块茎不定芽分化率进行对比。结果表明,‘夏坡蒂’、‘费乌瑞它’和‘克新1号’愈伤组织的最佳诱导培养基组合分别为MS+1 mg/L IAA+1 mg/L 6-BA+0.2 mg/L GA_3+1 mg/L ZT、MS+1 mg/L IAA+1 mg/L 6-BA+0.2 mg/L GA_3+2 mg/L ZT和MS+1 mg/L IAA+1 mg/L 6-BA+0.5 mg/L GA_3+2 mg/L ZT。‘夏坡蒂’与‘费乌瑞它’的不定芽最佳分化培养基组合为MS+1 mg/L IAA+1 mg/L 6-BA+0.5 mg/L GA_3+2 mg/L ZT;‘克新1号’的最佳分化培养基组合为MS+1 mg/L IAA+1 mg/L 6-BA+0.2 mg/L GA_3+2 mg/L ZT。试管薯的块茎不定芽分化率显著高于大田薯。
To establish the optimal potato tuber regeneration system, microtubers and field potato of‘Sharpdi＇,‘Favorite＇and‘Kexin No.1＇are used as the test materials. The regeneration system is screenedwith a controlled variable method to obtain the optimal hormone concentration of the medium. And acomparison of adventitious buds＇ differentiation rates of potato tubers from different sources is conducted.These results showed that the optimal callus induction mediums of‘Sharpdi＇,‘Favorite＇and‘Kexin No.1＇were MS＋1 mg/L IAA＋1 mg/L 6-BA＋0.2 mg/L GA3＋1 mg/L ZT, MS＋1 mg/L IAA＋1 mg/L 6-BA＋0.2 mg/LGA3＋2 mg/L ZT and MS＋1 mg/L IAA＋1 mg/L 6-BA＋0.5 mg/L GA3＋2 mg/L ZT, respectively. The optimaladventitious buds differentiation medium of‘Sharpdi＇and‘Favorite＇was MS＋1 mg/L IAA＋1 mg/L 6-BA＋0.5 mg/L GA3＋2 mg/L ZT; while for‘Kexin No.1＇, the optimal differentiation medium was MS＋1 mg/L IAA＋1 mg/L 6-BA ＋ 0.2 mg/L GA3 ＋ 2 mg/L ZT. The differentiation rate of adventitious buds of microtuber issignificantly higher than that of field potato.
作者
侯丁一
张之为
田再民
康立茹
赵君
Hou Dingyi;Zhang Zhiwei;Tian Zaimin;Kang Liru;Zhao Jun(College of Agronomy,Inner Mongolia Agriculture University,Hohhot 010018)
出处
《中国农学通报》
2018年第16期22-28,共7页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金"水杨酸(SA)在马铃薯小G蛋白St RAC介导的防卫反应体系建立过程中的功能初探"(31260425)
关键词
马铃薯
块茎
再生体系
愈伤组织
不定芽分化
potato
tuber
regeneration system
callus
adventitious bud differentiation
