摘要
为探明长期连作对大豆根际土壤微生物群落结构的影响,基于环境因子相同的黑土区大豆连作长期定位试验,采用实时定量聚合酶链式反应(Quantity-PCR,qPCR)和PCR-DGGE技术,解析大豆根际微生物群落丰度和结构组成在不同连作年限间的动态变化过程。结果表明,连作7年以上处理的细菌群落结构组成和多样性明显低于轮作和连作4年以下处理,由此可以看出,大豆连续种植导致土壤中细菌群落组成的演替,是根际作物-土壤-微生物相互作用缓慢累积的过程,直到连作7年才足以被检测出来。连作大豆根际真菌群落结构多样性分析结果表明,连作2年和连作4年根际真菌群落组成丰富,多样性较高。而连作7年以上和轮作处理的大豆根际真菌群落多样性指数为3. 067 7~3. 071 5,低于连作2年和连作4年处理,同时连作7年处理真菌群落组成与轮作处理相似性较高,由此可以看出大豆连作土壤被称作抑制性土壤的机制所在。
In order to explore the effects of long-term continuous cropping on the microbial community structures of soybean rhizosphere soil. Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis （PCR-DGGE） method was used to anal-ysis the dynamic process of soil microbial community based on long-term soybean continuous cropping experiment with the same environment factors. The results showed that soil bacteria community composition changed with continuous cropping years, where the diversity of bacteria community in soybean continuous cropping over seven years treatments were lower than that of rotation and continuous cropping less than four years, indicating that soil bacteria community was changed with soybean continuous cropping, but it is a slowly process. The results of fungi community structure analysis showed that the community composition of SC2 and SCA trend to more abundant and diverse. Then the diversities of fungi community for more than 7 years and the rotation treatment were from 3. 067 7 to 3.071 5, lower than that of SC2 and SCA, while the community composition of SC7 showed similar to rotation, which was the typical property of suppressive soil.
作者
陈雪丽
王玉峰
李伟群
王爽
王晓军
张磊
王英
CHEN Xue-li;WANG Yu-feng;LI Wei-qun;WANG Shuang;WANG Xiao-jun;ZHANG Lei;WANG Ying(Institute of Soil Fertilizer and Environment Resources, Key Laboratory of Soil Environment and Plant Nutrition of Heilongjiang Province, Fertilizer En-gineering Technology Research Centre of Heilongjiang Province, Heilongjiang Academy of Agricultural Sciences, Harbin 150056, China;Postdoc sta-tion of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China)
出处
《大豆科学》
CAS
CSCD
北大核心
2018年第5期748-755,共8页
Soybean Science
基金
国家重点研发计划子课题(2016YFD0300303)
国家科技重大专项(2015ZX07201-008)
中央引导地方科技发展专项(ZY18A04)
黑龙江省博士后资助项目(LBH-Z17198)
关键词
黑土
大豆连作
根际
微生物群落
多样性
Black soil
Soybean continuous cropping
Rhizosphere
Microbial community
Diversity of soil microbial
