摘要
目的:研究白术七物颗粒对结肠慢传输型便秘小鼠胃肠功能的影响及相关机制。方法:40只ICR小鼠以灌胃复方地芬诺酯法构建结肠慢传输型便秘模型,以10只灌胃等量生理盐水为空白组。将建模成功的38只小鼠随机分为4组,分别为模型组与对照组,每组9只,观察组低剂量组与高剂量组,每组10只。低/高剂量实验组分别予以白术七物颗粒生药量16.3 g/kg、65.2 g/kg灌胃,对照组予以2.0 g/kg枸橼酸莫沙必利溶液灌胃,模型组和空白组均灌胃等量生理盐水,1次/d,各组均连续干预14 d,末次给药的同时各组均给予1 m L纳米碳混悬液。干预结束后观察各组小鼠排便情况,测定肠道酶活性,免疫组化法检测结肠组织中5-羟色胺4(5-HT4)、血管活性肠肽(VIP)、一氧化氮(NO)、P物质(SP)表达情况。结果:与空白组比较,模型组小鼠第1次排黑便时间延长,排便粒数减少,粪便总重量降低,差异有统计学意义(P<0.05);与模型组比较,对照组和观察组低/高剂量实验组小鼠第1次排黑便的时间缩短,排便粒数增加,粪便总重量升高,差异有统计学意义(P<0.05);且低/高剂量实验组组间比较,差异有统计学意义(P<0.05)。与空白组比较,模型组小鼠肠道蛋白酶和纤维素酶活性降低,木聚糖酶活性升高,差异有统计学意义(P<0.05);与模型组比较,对照组与低剂量、高剂量实验组小鼠蛋白酶和纤维素酶活性升高,木聚糖酶活性降低,差异有统计学意义(P<0.05),且低/高剂量实验组组间比较,差异有统计学意义(P<0.05)。与空白组比较,模型组结肠组织中VIP、NO表达量增加,5-HT4、SP表达量减少,差异有统计学意义(P<0.05);与模型组比较,对照组和观察组低/高剂量实验组结肠组织中VIP、NO表达量减少,5-HT4、SP表达量增加,差异有统计学意义(P<0.05);且观察组低/高剂量实验组结肠组织中VIP、NO、5-HT4、SP表达量比较,差异有统计学意义(P<0.05)。结论:白术七
Objective： To investigate the effects of Baizhu Qiwu granule on gastrointestinal function in colon slow transit constipation mice and its related mechanisms. Methods： A total of 40 ICR mice were constructed colon slow transit constipation mice model by given compound diphenoxylate orally,and 10 cases of mice were given equal amount of normal saline orally as blank group. 38 cases of successful constipation models were randomly divided into model group（ n = 9）,positive control group（ n = 9） and low/high dose test group（ n = 10,test groups-L/H）. The test groups-L/H were given orally 16. 3 g/kg and 65. 2 g/kg respectively of Baizhu Qiwu granule raw medicine,and the positive control group was given orally 2 g/kg mosapride citrate tablets. The model group and the blank group were given equal amount of normal saline orally once daily for 14 d continuously,and 1 m L nano carbon suspension was given at the same time when the last administration was given in each group. After intervention,the defecation of mice in each group was observed,and the activities of intestinal enzyme were measured. Immunohistochemistry was used to detect the expression of 5-hydroxytryptamine 4（ 5-HT4）,vasoactive intestinal peptide（ VIP）,nitric oxide（ NO） and P substance（ SP）in colon tissue. Results： Compared with the blank group,the time of first time black stool in the model group prolonged,and the number of defecation particles decreased. The total weight of feces decreased（ P〈 0. 05）. Compared with the model group,the time of first time black stool in the positive control group and the test groups-L/H shortened,and the number of defecation particles increased. The total weight of feces increased（ P〈 0. 05）. There was significant difference between test group-L and test group-H（ P〈 0. 05）. Compared with the blank group,the activity of intestinal protease and cellulase of the model group decreased and the activity of xylanase increased（ P〈 0. 05）. Compared with the model group,the activi
作者
王满
刘妮妮
田萍
陈晓岚
Wang Man;Liu Nini;Tian Ping;Chen Xiaolan(Department of Anus and Intestine Surgery,Shenzhen the Second People＇s Hospital,Shenzhen 518032,China)
出处
《世界中医药》
CAS
2018年第8期1979-1983,共5页
World Chinese Medicine
基金
深圳市科技计划项目资助课题(JCYJ20140414170821332)
关键词
结肠慢传输型便秘
白术七物颗粒
胃肠功能
肠道酶活性
Colon slow transit constipation
Baizhu Qiwu granule
Gastrointestinal function
Intestinal enzyme activity
