摘要
目的探讨1-溴丙烷(1-BP)亚急性吸入染毒对雄性大鼠的大脑皮质、海马、小脑和脑干超微结构的影响。方法无特定病原体级雄性Wistar大鼠随机分为对照组和染毒组,每组6只。采用动式吸入染毒法,染毒组予质量浓度为5 000 mg/m3的1-BP气体染毒,对照组予吸入新鲜空气,6 h/d,5 d/周,连续4周。染毒结束后,采集大鼠大脑皮质、海马、小脑和脑干,透射电子显微镜下观察超微结构变化。结果染毒组大鼠在1-BP染毒第3周开始出现反应迟钝、后肢肌力下降现象;染毒组大鼠体质量在染毒第1~4周均低于同时间点对照组(P<0.05)。透射电子显微镜观察结果显示:染毒组大鼠的大脑皮质、海马区、小脑及脑干髓鞘结构均有破坏,神经元可见细胞核形状不规则、内质网空泡变性、溶酶体增多以及轴突内线粒体肿胀等表现,星形胶质细胞可见细胞核固缩,包绕血脑屏障的星形胶质细胞突起可见空泡化改变。结论 1-BP亚急性吸入染毒对雄性大鼠脑组织的髓鞘、神经元、星形胶质细胞和血脑屏障均可造成损害,以神经纤维的脱髓鞘改变和血脑屏障的通透性降低尤为明显。
Objective To explore the effects of sub-acute inhalation of 1-bromopropane（ 1-BP） on the ultrastructure of cerebral cortex,hippocampus,cerebellum,and brainstem in male rats. Methods Specific pathogen free healthy male Wistar rats were randomly divided into control group and exposure group with 6 rats in each group. The rats of exposure group received 1-BP vapor at a concentration of 5 000 mg/m3. The rats in the control group were given fresh air in a dynamic inhalation chamber system for 4 weeks（6 hours/day,5 days/week）. After the end of the exposure,the cerebral cortex,hippocampus,cerebellum and brainstem of rats were collected and the ultrastructural changes were observed under transmission electron microscope（ TEM）. Results After 3 weeks of exposure to 1-BP,the rats in the exposure group began to have unresponsiveness and decreased muscle strength in hind limbs. The body weight of exposure group was lower than that of control group from the 1 st to the 4 th week（ P 0. 05）. TEM results showed destroyed structure of the myelin sheath in the region of cerebral cortex, hippocampus, cerebellum and brainstem, and irregular nucleus, vacuolar degeneration,increased lysosome of endoplasmic reticulum,mitochondrion swelling of neuron cells,karyopyknosis of astrocytes and vacuolation in the neurite of astrocytes located in the blood brain barrier（ BBB）. Conclusion 1-BP sub-acute inhalation exposure could damage the myelin,neuron,astrocyte and BBB in male rats. The demyelination of nerve fiber and decreased permeability of BBB was particularly noticeable.
作者
伍津
宋向荣
邓莹玉
刘金华
谢植伟
赵娜
黄曼琪
王海兰
李宏玲
WU Jin;SONG Xiangrong;DENG Yingyu;LIU Jinhua;XIE Zhiwei;ZHAO Na;HUANG Manqi;WANG Hailan;LI Hongling(Guangdong Province Hospital for Occupational Disease Prevention and Treatment;Guangdong Provincial Key Laboratory of Occupational Disease Prevention and Treatment,Guangzhou,Guangdong 510300,China)
出处
《中国职业医学》
CAS
北大核心
2018年第4期424-429,共6页
China Occupational Medicine
基金
国家自然科学基金(81202246)
广东省医学科研基金(A2018152
A2016155
A2016458)
广东省科技计划项目(2016A020215131
2014A020212500)
关键词
1-溴丙烷
脑组织
超微结构
大脑皮质
海马
小脑
脑干
神经元
1-Bromopropane
Brain tissue
Ultrastructure
Cerebral cortex
Hippocampus
Cerebellum
Brainstem
Neuron
