摘要
为寻找植物源天然抗氧化剂,采用单因素试验、正交试验、葡聚糖凝胶柱层析纯化等方法对插田泡叶中SOD酶进行了研究,并对SOD酶的种类进行了初步鉴定。结果显示,插田泡叶中SOD酶的最佳提取条件是:插田泡叶与PBS物料比1︰3(g/mL)、PBS溶液pH 7.6、除杂饱和硫酸铵浓度45%、沉淀SOD饱和硫酸铵浓度90%。此条件下,SOD酶活为720.16 U。SOD粗酶液经葡聚糖凝胶柱层析纯化样品,初步鉴定为Mn-SOD。结果证实,插田泡叶含有SOD酶,这可为SOD新资源的开发及插田泡资源的综合利用提供科学依据。
For finding the natural anti-oxidants, purification and identification of superoxide dismutase from Rubus coreanusMaq. leaves were completed by the methods of one-factor-at-a-time experiments, orthogonal array design and sephadex column chromatography, et al. The results showed that the optimal extraction conditions were material/liquid ratio of 1：3（g/mL）,PBS solution（pH 7.6）, the concentration 45% of saturated ammonium sulfate for removing impurities and the concentration 90% of saturated ammonium sulfate for precipitation SOD protein. Under these conditions, the enzyme activity of SOD from Rubus coreanus Maq. leaves was 720.16 U. The purification sample by the sephadex column chromatography was indicated as Mn-SOD. In summary, Rubus coreanus Maq. leaves contain Mn-SOD. It could provide a scientific basis for the development of the new SOD resources and the comprehensive utilization of Rubus coreanus Maq. resource.
作者
蒋华梅
王向前
JIANG Huamei;WANG Xiangqian(College of Chemistry and Material Engineering,Guiyang University（Guiyang 550005;Library of Guiyang University（Guiyang 550005)
出处
《食品工业》
CAS
北大核心
2018年第9期81-85,共5页
The Food Industry
基金
贵州省科学技术基金项目:"插田泡中超氧化歧化酶(SOD)的提取纯化研究"(黔科合J字LKG[2013]13号)
贵州省高校优秀科技创新人才支持计划:"插田泡果实花色苷的分离纯化研究"(黔教合KY字[2015]500)
关键词
悬钩子属
插田泡
超氧化物歧化酶
提取
纯化
鉴定
Rubus. L
Rubus coreanus Maq.
superoxide dismutase
extraction
purification
identification
