摘要
目的:研究甜叶菊废渣提取物的抗氧化及抗炎作用。方法:甜叶菊废渣经提取、分离得到两种提取物(SWE-1和SWE-2)。采用DPPH法、ABTS法、FRAP法、ORAC法和饮食诱导小鼠氧化应激试验,评价甜叶菊废渣提取物的抗氧化活性;采用脂多糖(LPS)诱导小鼠巨噬细胞炎症模型、二甲苯致小鼠耳肿胀和角叉菜胶致小鼠足肿胀法,评价甜叶菊废渣提取物的抗炎活性。结果:甜叶菊废渣提取物能够清除多种自由基,具有较强的体外抗氧化活性;SWE-1可显著提高氧化应激小鼠血清总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活力,降低丙二醛(MDA)的含量。甜叶菊废渣提取物还能显著抑制LPS诱导的巨噬细胞NO释放量,具有良好的抗炎活性;SWE-1能抑制二甲苯致耳肿胀和角叉菜胶致足肿胀,并能降低血清中炎症因子NO的含量。结论:甜叶菊废渣提取物具有较强的体内外抗氧化和抗炎作用,具有广阔的开发和利用前景,可用于功能性食品和保健品。
Objective： The objective of the study was to evaluate the antioxidant and anti-inflammatory activity of Stevia rebaudiana waste extract. Methods： Two different extracts （SWE-1 and SWE-2） were obtained from Stevia rebau- dianna waste by extraction and isolation. The antioxidant activity of Stevia rebaudiana waste extract was evaluated by DPPH, ABTS, FRAP, ORAC, and diet induced mice oxidative stress assays. The anti-inflammatory activity of Stevia rebaudiana waste extract was evaluated by lipopolysaccharide-induced murine maerophage inflammatory, dimethylbenzene induced ear swelling, and carrageenan induced paw edema assays. Results： Stevia rebaudiana waste extract could scav- enge a variety of free radicals, showing strong antioxidant activity in vitro. SWE-1 significantly increased the activity of serum T-AOC, SOD and decreased the content of MDA in oxidative stress mice. Stevia rebaudiana waste extract signifi- cantly inhibited NO production in LPS induced maerophages, showing good anti-inflammatory activities. SWE-1 could in- hibit dimethylbenzene induced ear swelling and carrageenan induced paw edema in mice, also decrease the inflammatory factor content of NO. Conclusion： Stevia rebaudiana waste extract had strong antioxidant and anti-inflammatory activities in vitro and in vivo. With a good prospect of development and utilization, it could be used as a functional food or nu- traceutieal product.
作者
赵磊
迟茜
林文轩
王成涛
徐美利
连运河
杨香瑜
李乾丽
Zhao Lei;Chi Qian;Lin Wenxuan;Wang Chengtao;Xu Meili;Lian Yunhe;Yang Xiangyu;Li Qianli(Beijing Technology and Business University,Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Engineering and Technology Research Center of Food Additives,Beijing 100048 2Chenguang Biotech Group Co.,Ltd.,Handan 057250,Hebei)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2018年第8期8-15,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
"十三五"北京市属高校高水平教师队伍建设支持计划(CIT&TCD201704042)
北京市科技计划项目(Z171100002217019)
国家重点研发计划项目(2016YFD0400802)
北京市属高校创新能力提升计划项目(0142132016)
关键词
甜叶菊
抗氧化
抗炎
氧化应激
巨噬细胞
Stevia rebaudiana
antioxidant
anti-inflammatory
oxidative stress
macrophage
