摘要
目的考察豆蔻佛波醇乙酯(PMA)对体外培养的原单核细胞THP-1的分化诱导条件。方法首先用PMA梯度剂量(0,25,50,100,200,400 ng/ml)作用于THP-1细胞24 h,通过CCK-8法检测细胞活力,再分别采用0,10,50 ng/ml PMA体外刺激THP-1细胞24 h、48 h,流式细胞术检测细胞表面标志物CD11b、CD14。结果梯度PMA剂量(0-100 ng/ml)对细胞活力没有影响(P>0.05)。10 ng/ml、50 ng/ml PMA处理后,CD11b、CD14阳性细胞百分比均有显著上调(与0 ng/ml相比,P<0.05)。结论 10 ng/ml PMA能够诱导THP-1细胞向巨噬细胞方向分化,为PMA诱导THP-1细胞分化为巨噬细胞模型提供依据。
Objective To investigate the differentiation induction conditions of phorbol myristate acetate（PMA） of in vitro cultured promonocytes THP-1.Methods THP-1 cells were first treated with PMA gradient doses（0,25,50,100,200,400 ng/ml） for 24 h, and the cell viability was measured by CCK-8 assay. Then in vitro THP-1 cells were stimulated by 0,10, 50 ng/ml PMA respectively for 24 h and 48 h. Flow cytometry was used to detect cell surface markers CD11b and CD14.Results The gradient PMA dose （0-100 ng/ml） had no effect on cell viability（ P 〉0.05）.After 10 ng/ml and 50 ng/ml PMA treatment, the percentage of CD11b and CD14 positive cells was significantly increased（ P 〈0.05 vs 0 ng/ml）.Conclusion The 10 ng/ml PMA can induce THP-1 cells to differentiate towards macrophage, which provides a basis for PMA-induced differentiation of THP-1 cells into macrophage models.
作者
李晓琴
陈利荣
LI Xiaoqin;CHEN Lirong(Department of Medical Laboratory Science,Fenyang College of Shanxi Medical University,Fenyang 032200,China)
出处
《山西医科大学学报》
CAS
2018年第9期1051-1054,共4页
Journal of Shanxi Medical University
