摘要
为了研究沙苑子提取物对运动训练大鼠脑组织的保护作用及对相关生理生化指标的影响,选取成年雄性SD大鼠30只,适应性饲养、筛选,淘汰个别不适应跑台训练者。将剩余大鼠随机分为3组:安静对照组8只,运动对照组8只,运动加药组8只。进行8周大强度耐力跑台训练,测定了大鼠脑组织中超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量、ATP酶活性,一氧化氮(NO)和一氧化氮合酶(iNOS)等指标。结果表明:服用沙苑子提取物的大鼠脑组织的GSH-Px、CAT和SOD活性显著高于对照组,MDA含量显著下降,ATP酶活性增强,一氧化氮含量升高且一氧化氮合酶活性增强。补充沙苑子可显著减轻大强度耐力训练对大鼠脑组织造成的脂质过氧化产生的自由基对机体的损伤,有利于保护细胞膜的完整性,提高运动能力。
The protective affect of Astragalus complanatus extract on the brain tissue in exercise training rats and the physiological and biochemical indicators were investigated.Thirty adult male sprague-dawley rats were selected reared,screened and eliminated individually.The remaining rats were randomly divided into 3 groups,that is the quiet control group of 8 rats（A）,exercise control group of 8 rats（B）,exercise dosing group of 8 rats（C）.After 8 weeks of intensive endurance treadmill training,the activities of superoxide dismutase（SOD）,catalase（CAT）and glutathione peroxidase（GSH-Px）,malondialdehyde（MDA）content,ATPase activity,nitric oxide and nitric oxide synthase were tested.The results show that the activities of GSH-Px,CAT andSOD in brain tissue of rats treated with aconite root extract were significantly higher than those of the control group.The content of MDA was significantly decreased and the activity of ATPase increased mean while the content of nitric oxide and the activity of nitric oxide synthase were increased.Astragalus complanatus supplementation can significantly reduce the damage of free radicals produced by lipid peroxidation induced by high-intensity endurance training in rats and protect the integrity of cell membranes and exercise ability.
作者
王轲
张晓琴
刘军
徐耀铎
WANG Ke;ZHANG Xiaoqin;LIU Jun;XU Yaoduo(Department of Physical Education,Northwest Polytechnical University,Xi＇an 710072,Shaanxi,China;Department of Health Sciences,Xi＇an Physical Education University,Xi＇an 710068,Shaanxi,China)
出处
《陕西师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第5期120-124,共5页
Journal of Shaanxi Normal University：Natural Science Edition
基金
陕西省自然科学基金(2012JM4014)
关键词
沙苑子提取物
运动训练
脑组织
大鼠
astragalus complanatus
exercise training
brain tissue
rats
