摘要
目的:利用气相色谱法测定莲藕渣中多糖的单糖组分。方法:通过水提醇沉法从莲藕渣中提取多糖,将提取的多糖经纯化、水解、衍生化后,利用气相色谱对其单糖组分进行分析。结果:水提醇沉法得到莲藕渣粗多糖LRP,得率为2.68%;经分离纯化得莲藕渣多糖LRP1和LRP2,其单糖组成为鼠李糖、阿拉伯糖、葡萄糖、木糖、半乳糖和岩藻糖,且其摩尔比为5.32∶16.03∶5.14∶1.02∶25.98∶2.32。结论:莲藕渣多糖主要由半乳糖和阿拉伯糖组成,其中半乳糖的含量最高,为25.98%。
Objective：To determine the monosaccharide composition of polysaccharides in lotus root residues by gas chromatography.Methods：Polysaccharides are extracted from lotus root residues by water extraction and alcohol precipitation method.The extracted polysaccharides are purified,hydrolyzed and derivatized.The components of monosaccharides are analyzed by gas chromatography.Results：The water-alcohol precipitation method is used to obtain the LRP with the yield of 2.68%.The LRP1 and LRP2 of the lotus root polysaccharides are separated and purified,and the monosaccharide components are rhamnose,arabinose,glucose,xylose,galactose and fucose,and the molar ratios are 5.32∶16.03∶5.14∶1.02∶25.98∶2.32.Conclusion：The lotus root polysaccharides mainly consist of galactose and arabinose with the highest content of galactose of 25.98%.
作者
孙丰婷
孙风光
李威
SUN Feng-ting;SUN Feng-guang;LI Wei(College of Food Science and Engineering,Yangzhou University,Yangzhou 225127,China;Jiangsu Sinography Testing Co.,Ltd.,Nanjing 210061,China;School of Life Science,Huaiyin Normal University,Huai＇an 223300,China;Shandong Animal Husbandry and Veterinary College.Weifang 261000,China)
出处
《中国调味品》
CAS
北大核心
2018年第9期144-146,共3页
China Condiment
关键词
莲藕渣
单糖
气相色谱
lotus root residue
monosaccharide
gas chromatography
